RRC ID 59997
著者 Sharma N, Baek K, Shimokawa N, Takagi M.
タイトル Effect of temperature on raft-dependent endocytic cluster formation during activation of Jurkat T cells by concanavalin A.
ジャーナル J Biosci Bioeng
Abstract Temperature plays an important role in the immune response. Acclimatization occurs when there are changes in ambient temperature over a long period. In this study, we used the human leukemic Jurkat T cell line to study the effect of temperature on the immune system using concanavalin A (ConA), a plant-derived immunostimulant, as a trigger for T-cell activation. Previously, we have reported endocytic intracellular cluster formation during T-cell activation by ConA with the aid of rafts and polymerization of the cytoskeleton (actin and microtubules). Here, we investigated the effect of temperature on cluster formation (with the aid of three-dimensional images of the cells) and on the stability of rafts, actin, and microtubules. When the temperature was changed between 23°C and 37°C (physiological temperature), clusters could be observed throughout this temperature range. Raft structure was stabilized at lower temperatures but destabilized at higher temperatures. Actin was stable when the temperature was higher than 27°C. When actin was depolymerized, clustering was not observed at 37°C but could be observed at 23°C. There were no changes in microtubules within this temperature range. Thus, raft clustering may be associated with raft stability at lower temperatures (<27°C) and with actin at higher temperatures (≥27°C). Hence, we provided insight into the associations between temperature, rafts, actin, and microtubules in the immune response.
巻・号 127(4)
ページ 479-485
公開日 2019-4-1
DOI 10.1016/j.jbiosc.2018.09.014
PII S1389-1723(18)30670-4
PMID 30355461
MeSH Actins / drug effects Actins / metabolism Concanavalin A / pharmacology* Cytoskeleton / drug effects Cytoskeleton / metabolism Endocytosis / drug effects Humans Jurkat Cells Lymphocyte Activation / drug effects* Membrane Microdomains / drug effects Membrane Microdomains / physiology* Microtubules / drug effects Microtubules / metabolism Protein Multimerization / drug effects Temperature* Transport Vesicles / drug effects* Transport Vesicles / metabolism
IF 2.032
引用数 0
リソース情報
ヒト・動物細胞 Jurkat(RCB0806)