RRC ID |
60023
|
著者 |
Mochizuki M, Nakamura M, Sibuya R, Okazaki T, Abe J, Nakagawa T, Takahashi S, Yamazaki T, Imai T, Takano A, Ito H, Yokose T, Miyagi Y, Daigo Y, Sato I, Satoh K, Sugamura K, Yamaguchi K, Tamai K.
|
タイトル |
CD271 is a negative prognostic factor and essential for cell proliferation in lung squamous cell carcinoma.
|
ジャーナル |
Lab Invest
|
Abstract |
Squamous cell carcinoma is a major type of cancer in the lung. While several therapeutic target molecules for lung adenocarcinoma have been identified, little is known about lung squamous cell carcinoma (LSCC). We recently reported that CD271 (p75 neurotrophin receptor) serves as a marker for tumor initiation and is a key regulator of cell proliferation in hypopharyngeal squamous cell carcinoma. In this study, we found that CD271 was also expressed in squamous cell carcinoma, but not in adenocarcinoma, of several tissues, including the lung, and the expression of CD271 was associated with a poor prognosis in LSCC. To examine CD271's role in LSCC, we established xenograft cell lines from LSCC patients. Within the sorted live LSCC cell population, the CD271high cells were primarily cycling through the G2/M phase, while the CD271low cells were mostly in the G0 phase. CD271 knockdown in the LSCC cells completely suppressed their proliferation and tumor-formation capability, and increased their cell-cycle arrest in the G0 phase. In the CD271-knockdown cells, ERK-phosphorylation was decreased, while no change was observed in the IκBα-phosphorylation, p65-phosphorylation, or Akt-phosphorylation. Treatment with the MEK inhibitor U0126 decreased the LSCC cells' proliferation capability. Microarray analysis revealed that CD271 knockdown attenuated the RAS-related pathways. The knockdown of TrkB, which forms a heterodimer with CD271 and accelerates its downstream signaling, partially inhibited the LSCC cell proliferation. These results indicated that LSCC exclusively depends on CD271 for cell proliferation, in part through ERK-signaling activation, and CD271 is a promising target for LSCC therapy.
|
巻・号 |
99(9)
|
ページ |
1349-1362
|
公開日 |
2019-9-1
|
DOI |
10.1038/s41374-019-0246-5
|
PII |
10.1038/s41374-019-0246-5
|
PMID |
31019292
|
MeSH |
Aged
Aged, 80 and over
Animals
Biomarkers, Tumor
Carcinoma, Squamous Cell / diagnosis
Carcinoma, Squamous Cell / metabolism*
Carcinoma, Squamous Cell / mortality
Cell Line, Tumor
Cell Movement / genetics
Cell Proliferation / genetics*
Disease-Free Survival
Female
Humans
Lung Neoplasms / diagnosis
Lung Neoplasms / metabolism*
Lung Neoplasms / mortality
Male
Mice, Inbred NOD
Mice, SCID
Middle Aged
Nerve Tissue Proteins* / genetics
Nerve Tissue Proteins* / metabolism
Prognosis
Receptors, Nerve Growth Factor* / genetics
Receptors, Nerve Growth Factor* / metabolism
|
IF |
3.684
|
引用数 |
2
|
リソース情報 |
ヒト・動物細胞 |
LK-2(RCB1970) |