RRC ID 60029
著者 Jin C, Zhao W, Zhang Z, Liu W.
タイトル Silencing circular RNA circZNF609 restrains growth, migration and invasion by up-regulating microRNA-186-5p in prostate cancer.
ジャーナル Artif Cells Nanomed Biotechnol
Abstract Background: Prostate cancer (PC) fearfully impacts men's health. We explored the efficacy and mechanism of circular RNA circZNF609 (circZNF609) on colony formation, viability, apoptosis, migration and invasion and in PC cells. Methods: Colony formation, CCK-8, flow cytometry, migration and invasion assay were respectively used to detect the functions of circZNF609 and microRNA (miR)-186-5p on cell colony ability, viability, apoptosis, migration and invasion. circZNF609 and miR-186-5p expression were changed by cell transfection and tested by RT-qPCR. Moreover, Cleaved-Caspase-3, Cleaved-Caspase-9, matrix metalloproteinase-9 (MMP-9), Vimentin and relate-proteins of cell pathways were examined through Western blot. Results: circZNF609 was highly expressed at PC tissues. circZNF609 declined cell colony ability, viability, migration and invasion and caused apoptosis. Furthermore, circZNF609 negatively regulated miR-186-5p, miR-186-5p inhibitor could reverse impacts of circZNF609. Finally, circZNF609 restrained the YAP1 and AMPK pathways by up-regulating miR-186-5p. Conclusion: Silencing circZNF609 restrained growth, migration and invasion of PC cells by up-regulating miR-186-5p via YAP1 and AMPK pathways. Highlights circZNF609 is highly expressed in PC tissues; circZNF609 restrains cell growth, migration and invasion; circZNF609 exerts its function by up-regulating miR-186-5p; circZNF609 exerts its function by YAP1 and AMPK signaling pathways.
巻・号 47(1)
ページ 3350-3358
公開日 2019-12-1
DOI 10.1080/21691401.2019.1648281
PMID 31387394
MeSH AMP-Activated Protein Kinases / metabolism Adaptor Proteins, Signal Transducing / metabolism Cell Movement / genetics* Cell Proliferation / genetics Humans Male MicroRNAs / genetics* Neoplasm Invasiveness / genetics PC-3 Cells Prostatic Neoplasms / genetics Prostatic Neoplasms / pathology* RNA Interference* RNA, Circular / deficiency* RNA, Circular / genetics* Signal Transduction / genetics Transcription Factors / metabolism Up-Regulation / genetics* YAP-Signaling Proteins
IF 4.462
引用数 6
リソース情報
ヒト・動物細胞 LNCap.FGC(RCB2144)