RRC ID 60038
Author Miyamoto H, Sawaji Y, Iwaki T, Masaoka T, Fukada E, Date M, Yamamoto K.
Title Intermittent pulsed electromagnetic field stimulation activates the mTOR pathway and stimulates the proliferation of osteoblast-like cells.
Journal Bioelectromagnetics
Abstract Pulsed electromagnetic fields (PEMFs) have been shown to be a noninvasive physical stimulant for bone fracture healing. However, PEMF stimulation requires a relatively long period of time and its mechanism of action has not yet been fully clarified. Recently, the mammalian target of rapamycin (mTOR) pathway has been shown to be involved in bone formation. This study aimed to investigate the effects of PEMFs on osteoblastic MC3T3-E1 cells by examining various cellular responses including changes in the mTOR pathway. Continuous PEMF stimulation induced a transient phosphorylation of the mTOR pathway, whereas intermittent PEMF stimulation (1 cycle of 10 min stimulation followed by 20 min of stimulation pause) revitalized the reduced phosphorylation. Moreover, PEMF stimulation stimulated cell proliferation (bromodeoxyuridine incorporation) rather than differentiation (alkaline phosphatase activity), with a more notable effect in the intermittently stimulated cells. These results suggest that intermittent PEMF stimulation may be effective in promoting bone fracture healing by accelerating cell proliferation, and in shortening stimulation time. Bioelectromagnetics. 2019;40:412-421. © 2019 Bioelectromagnetics Society.
Volume 40(6)
Pages 412-421
Published 2019-9-1
DOI 10.1002/bem.22207
PMID 31338867
MeSH Alkaline Phosphatase / metabolism Bromodeoxyuridine / metabolism Cell Line Cell Proliferation Electric Stimulation Electromagnetic Fields* Humans Osteoblasts / cytology* Phosphorylation Signal Transduction TOR Serine-Threonine Kinases / metabolism*
IF 1.945
Times Cited 0
Human and Animal Cells MC3T3-E1(RCB1126)