RRC ID 6007
Author Iguchi F, Nakagawa T, Tateya I, Kim TS, Endo T, Taniguchi Z, Naito Y, Ito J.
Title Trophic support of mouse inner ear by neural stem cell transplantation.
Journal Neuroreport
Abstract In the auditory system, efforts to reduce degeneration of spiral ganglion neurons have the immediate objective of improving clinical benefits of cochlear implants, which are small devices designed to stimulate spiral ganglion neurons electronically. Recent studies have indicated several neurotrophins can enhance survival of spiral ganglion neurons. However, the strategy for application of neurotrophins in inner ear is still a matter of debate. In this study, we examined the potential of cell therapy as a strategy for application of neurotrophins in the inner ear. Neural stem cells obtained from green fluorescent protein-transgenic mice were used as donor cells. Medium containing neural stem cells was injected into mouse inner ear. Histological analysis 4 weeks later revealed that transplant-derived cells survived in inner ear and that most transplant-derived cells in the cochlea had differentiated into glial cells. Moreover, expression of glial cell line-derived neurotrophic factor and brain-derived neurotrophic factor was observed in transplant-derived cells. These findings indicate that transplantation of neural stem cells can be a useful strategy for application of neurotrophins in inner ear.
Volume 14(1)
Pages 77-80
Published 2003-1-20
DOI 10.1097/01.wnr.0000050714.17082.9b
PMID 12544835
MeSH Animals Brain Tissue Transplantation* Brain-Derived Neurotrophic Factor / analysis Cell Differentiation Cell Lineage Cochlea / chemistry Cochlea / cytology* Female Fetal Tissue Transplantation* Glial Cell Line-Derived Neurotrophic Factor Glial Fibrillary Acidic Protein / analysis Graft Survival Green Fluorescent Proteins Luminescent Proteins / analysis Luminescent Proteins / genetics Mice Mice, Inbred C57BL Mice, Transgenic Microtubule-Associated Proteins / analysis Nerve Growth Factors / analysis Nerve Tissue Proteins / analysis Neuroglia / chemistry Neuroglia / cytology Neurons / chemistry Neurons / cytology Semicircular Canals Stem Cell Transplantation* Telencephalon / cytology Telencephalon / embryology Transplantation, Heterotopic*
IF 1.266
Times Cited 68
WOS Category NEUROSCIENCES
Resource
Mice C57BL/6-TgN(act-EGFP)OsbC14-Y01-FM131