| RRC ID |
60083
|
| 著者 |
Islam I, Baba Y, Witarto AB, Yoshida W.
|
| タイトル |
G-quadruplex-forming GGA repeat region functions as a negative regulator of the Ccnb1ip1 enhancer.
|
| ジャーナル |
Biosci Biotechnol Biochem
|
| Abstract |
An enhancer located upstream of the transcriptional start site of Ccnb1ip1 containing two GGA-rich regions and a 14-GGA repeat (GGA)14 region has been previously identified. Three copies of four GGA repeats in the c-myb promoter that form a tetrad:heptad:heptad:tetrad (T:H:H:T) dimerized G-quadruplex (G4) structure reportedly functions as both a transcriptional repressor and activator. Here, the secondary structures of the two GGA-rich and (GGA)14 regions were analyzed using circular dichroism spectral analysis, which indicated that the two GGA-rich DNAs formed parallel-type G4 structures, whereas (GGA)14 DNA formed the T:H:H:T dimerized G4 structure. Reporter assays demonstrated that individual regions did not show enhancer activity; however, the deletion of the (GGA)14 region resulted in 1.5-fold higher enhancer activity than that of the whole enhancer. These results indicate that the (GGA)14 region that forms the T:H:H:T dimerized G4 structure functions as a negative regulator of the Ccnb1ip1 enhancer.
|
| 巻・号 |
83(9)
|
| ページ |
1697-1702
|
| 公開日 |
2019-9-1
|
| DOI |
10.1080/09168451.2019.1611412
|
| PMID |
31062645
|
| MeSH |
Adaptor Proteins, Signal Transducing / genetics*
Animals
Cell Cycle Proteins / genetics*
Circular Dichroism
Enhancer Elements, Genetic*
G-Quadruplexes*
Humans
Mice
NIH 3T3 Cells
Repetitive Sequences, Nucleic Acid*
Ubiquitin-Protein Ligases
|
| IF |
1.297
|
| 引用数 |
1
|
| リソース情報 |
| ヒト・動物細胞 |
NIH3T3-3-4(RCB1862) |
