RRC ID 60083
Author Islam I, Baba Y, Witarto AB, Yoshida W.
Title G-quadruplex-forming GGA repeat region functions as a negative regulator of the Ccnb1ip1 enhancer.
Journal Biosci Biotechnol Biochem
Abstract An enhancer located upstream of the transcriptional start site of Ccnb1ip1 containing two GGA-rich regions and a 14-GGA repeat (GGA)14 region has been previously identified. Three copies of four GGA repeats in the c-myb promoter that form a tetrad:heptad:heptad:tetrad (T:H:H:T) dimerized G-quadruplex (G4) structure reportedly functions as both a transcriptional repressor and activator. Here, the secondary structures of the two GGA-rich and (GGA)14 regions were analyzed using circular dichroism spectral analysis, which indicated that the two GGA-rich DNAs formed parallel-type G4 structures, whereas (GGA)14 DNA formed the T:H:H:T dimerized G4 structure. Reporter assays demonstrated that individual regions did not show enhancer activity; however, the deletion of the (GGA)14 region resulted in 1.5-fold higher enhancer activity than that of the whole enhancer. These results indicate that the (GGA)14 region that forms the T:H:H:T dimerized G4 structure functions as a negative regulator of the Ccnb1ip1 enhancer.
Volume 83(9)
Pages 1697-1702
Published 2019-9-1
DOI 10.1080/09168451.2019.1611412
PMID 31062645
MeSH Adaptor Proteins, Signal Transducing / genetics* Animals Cell Cycle Proteins / genetics* Circular Dichroism Enhancer Elements, Genetic* G-Quadruplexes* Humans Mice NIH 3T3 Cells Repetitive Sequences, Nucleic Acid* Ubiquitin-Protein Ligases
IF 1.297
Times Cited 1
Human and Animal Cells NIH3T3-3-4(RCB1862)