論文 - 詳細
| RRC ID | 60083 |
|---|---|
| 著者 | Islam I, Baba Y, Witarto AB, Yoshida W. |
| タイトル | G-quadruplex-forming GGA repeat region functions as a negative regulator of the Ccnb1ip1 enhancer. |
| ジャーナル | Biosci Biotechnol Biochem |
| Abstract |
An enhancer located upstream of the transcriptional start site of Ccnb1ip1 containing two GGA-rich regions and a 14-GGA repeat (GGA)14 region has been previously identified. Three copies of four GGA repeats in the c-myb promoter that form a tetrad:heptad:heptad:tetrad (T:H:H:T) dimerized G-quadruplex (G4) structure reportedly functions as both a transcriptional repressor and activator. Here, the secondary structures of the two GGA-rich and (GGA)14 regions were analyzed using circular dichroism spectral analysis, which indicated that the two GGA-rich DNAs formed parallel-type G4 structures, whereas (GGA)14 DNA formed the T:H:H:T dimerized G4 structure. Reporter assays demonstrated that individual regions did not show enhancer activity; however, the deletion of the (GGA)14 region resulted in 1.5-fold higher enhancer activity than that of the whole enhancer. These results indicate that the (GGA)14 region that forms the T:H:H:T dimerized G4 structure functions as a negative regulator of the Ccnb1ip1 enhancer. |
| 巻・号 | 83(9) |
| ページ | 1697-1702 |
| 公開日 | 2019-9-1 |
| DOI | 10.1080/09168451.2019.1611412 |
| PMID | 31062645 |
| MeSH | Adaptor Proteins, Signal Transducing / genetics* Animals Cell Cycle Proteins / genetics* Circular Dichroism Enhancer Elements, Genetic* G-Quadruplexes* Humans Mice NIH 3T3 Cells Repetitive Sequences, Nucleic Acid* Ubiquitin-Protein Ligases |
| IF | 1.297 |
| 引用数 | 1 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 3 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | NIH3T3-3-4(RCB1862) |