論文 - 詳細
| RRC ID | 60089 |
|---|---|
| 著者 | Zeng J, Tang SY, Wang S. |
| タイトル | Derivation of mimetic γδ T cells endowed with cancer recognition receptors from reprogrammed γδ T cell. |
| ジャーナル | PLoS One |
| Abstract |
Using induced pluripotent stem cells (iPSCs) to derive chimeric antigen receptor-modified T (CAR-T) cells has great industrial potential. A previous study used αβ T cell-derived CAR-modified iPSCs to produce CAR-T cells. However, these αβ T cells are restricted to autologous use and only recognize single cancer antigen. To make CAR-T alternative for allogeneic use, we reprogrammed γδ T cell into iPSCs (γδ T-iPSCs) to circumvent the risk of graft-versus-host disease. To target multiple cancer-associated antigens, we used an "NK cell-promoting" protocol to differentiate γδ T-iPSCs and to induce expression of natural killer receptors (NKRs). Through such two-step strategy, mimetic γδ T cells endowed with an array of NKRs and thus designated as "γδ natural killer T (γδ NKT) cells" were derived. With no/low-level expression of inhibitory killer cell immunoglobulin-like receptors (KIRs) and immune checkpoint receptors, γδ NKT cells may provide a potent "off-the-shelf" cytotoxic cell source to recognize multiple ubiquitous antigens in a broad spectrum of cancers. |
| 巻・号 | 14(5) |
| ページ | e0216815 |
| 公開日 | 2019-1-1 |
| DOI | 10.1371/journal.pone.0216815 |
| PII | PONE-D-18-34048 |
| PMID | 31071196 |
| PMC | PMC6508724 |
| MeSH | Antigens, Neoplasm / genetics Antigens, Neoplasm / immunology* HCT116 Cells Hep G2 Cells Humans Induced Pluripotent Stem Cells / immunology* Induced Pluripotent Stem Cells / pathology K562 Cells MCF-7 Cells Natural Killer T-Cells / immunology* Natural Killer T-Cells / pathology Neoplasms / genetics Neoplasms / immunology* Receptors, Antigen, T-Cell, alpha-beta / genetics Receptors, Antigen, T-Cell, alpha-beta / immunology Receptors, Antigen, T-Cell, gamma-delta / immunology* Receptors, Chimeric Antigen / genetics Receptors, Chimeric Antigen / immunology* THP-1 Cells |
| IF | 2.776 |
| 引用数 | 1 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 14 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 6.0 |
| リソース情報 | |
| ヒト・動物細胞 | OP9-DLL1 |