| RRC ID |
60089
|
| 著者 |
Zeng J, Tang SY, Wang S.
|
| タイトル |
Derivation of mimetic γδ T cells endowed with cancer recognition receptors from reprogrammed γδ T cell.
|
| ジャーナル |
PLoS One
|
| Abstract |
Using induced pluripotent stem cells (iPSCs) to derive chimeric antigen receptor-modified T (CAR-T) cells has great industrial potential. A previous study used αβ T cell-derived CAR-modified iPSCs to produce CAR-T cells. However, these αβ T cells are restricted to autologous use and only recognize single cancer antigen. To make CAR-T alternative for allogeneic use, we reprogrammed γδ T cell into iPSCs (γδ T-iPSCs) to circumvent the risk of graft-versus-host disease. To target multiple cancer-associated antigens, we used an "NK cell-promoting" protocol to differentiate γδ T-iPSCs and to induce expression of natural killer receptors (NKRs). Through such two-step strategy, mimetic γδ T cells endowed with an array of NKRs and thus designated as "γδ natural killer T (γδ NKT) cells" were derived. With no/low-level expression of inhibitory killer cell immunoglobulin-like receptors (KIRs) and immune checkpoint receptors, γδ NKT cells may provide a potent "off-the-shelf" cytotoxic cell source to recognize multiple ubiquitous antigens in a broad spectrum of cancers.
|
| 巻・号 |
14(5)
|
| ページ |
e0216815
|
| 公開日 |
2019-1-1
|
| DOI |
10.1371/journal.pone.0216815
|
| PII |
PONE-D-18-34048
|
| PMID |
31071196
|
| PMC |
PMC6508724
|
| MeSH |
Antigens, Neoplasm / genetics
Antigens, Neoplasm / immunology*
HCT116 Cells
Hep G2 Cells
Humans
Induced Pluripotent Stem Cells / immunology*
Induced Pluripotent Stem Cells / pathology
K562 Cells
MCF-7 Cells
Natural Killer T-Cells / immunology*
Natural Killer T-Cells / pathology
Neoplasms / genetics
Neoplasms / immunology*
Receptors, Antigen, T-Cell, alpha-beta / genetics
Receptors, Antigen, T-Cell, alpha-beta / immunology
Receptors, Antigen, T-Cell, gamma-delta / immunology*
Receptors, Chimeric Antigen / genetics
Receptors, Chimeric Antigen / immunology*
THP-1 Cells
|
| IF |
2.776
|
| 引用数 |
1
|
| リソース情報 |
| ヒト・動物細胞 |
OP9-DLL1 |
