論文 - 詳細
| RRC ID | 60225 |
|---|---|
| 著者 | Wang Y, Cao P, Sun P, Zhao J, Sun X, Zhang J, Li C, Xiang W, Wang X. |
| タイトル | Cryobacterium tepidiphilum sp. nov., isolated from rhizosphere soil of lettuce (var. ramosa Hort.). |
| ジャーナル | Antonie Van Leeuwenhoek |
| Abstract |
A Gram-stain positive, aerobic, rod-shaped bacterium, designated strain NEAU-85T, was isolated from rhizosphere soil of lettuce and characterised using a polyphasic approach. Strain NEAU-85T was found to be catalase positive, motile and able to grow at between 10 and 30 °C. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain NEAU-85T belongs to the genus Cryobacterium and is closely related to the type strain Cryobacterium psychrotolerans JCM 13925T (98.5%) and also to Leifsonia kafniensis NCCB 100216T (97.6%). Multilocus sequence analysis using the concatenated gene sequences of the atpD, dnaK, recA, rpoB, sevY and ychF genes showed that strain NEAU-85T belongs to the genus Cryobacterium. The digital DNA-DNA hybridization values revealed that strain NEAU-85T is distinct from its close phylogenetic neighbour C. psychrotolerans. The menaquinones were identified as MK-10 and MK-11. The phospholipid profile was found to consist of diphosphatidylglycerol, phosphatidylglycerol, phospholipid, an unidentified glycolipid and an unidentified lipid. The major fatty acids were identified as anteiso-C15:0, anteiso-C15:1 and iso-C16:0. The genomic DNA G + C content of strain NEAU-85T was determined to be 68.9 mol%. The DNA-DNA hybridization value between them was less than 70%. On the basis of phenotypic, genotypic and phylogenetic data, strain NEAU-85T can be concluded to represent a novel species of the genus Cryobacterium, for which the name Cryobacterium tepidiphilum sp. nov. is proposed. The type strain is NEAU-85T (= CCTCC AA 2018035T = JCM 32545T). |
| 巻・号 | 112(11) |
| ページ | 1611-1621 |
| 公開日 | 2019-11-1 |
| DOI | 10.1007/s10482-019-01287-3 |
| PII | 10.1007/s10482-019-01287-3 |
| PMID | 31222464 |
| MeSH | Actinobacteria / chemistry Actinobacteria / classification* Actinobacteria / genetics Actinobacteria / isolation & purification* Bacterial Typing Techniques Base Composition Genome, Bacterial Lactuca / microbiology* Multilocus Sequence Typing Phenotype Phylogeny RNA, Ribosomal, 16S / genetics Rhizosphere* Soil Microbiology* |
| IF | 1.934 |
| 引用数 | 0 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | Blogs |
| 各媒体での言及数の合計 | 2 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 一般微生物 | JCM13925 JCM32545 |