論文 - 詳細
| RRC ID | 60295 |
|---|---|
| 著者 | Horie A, Tomita Y, Ohshio K, Fujiwara D, Fujii T. |
| タイトル | Characterization of genomic DNA of lactic acid bacteria for activation of plasmacytoid dendritic cells. |
| ジャーナル | BMC Microbiol |
| Abstract |
BACKGROUND:Lactococcus lactis strain Plasma (LC-Plasma) possesses strong stimulatory activity for plasmacytoid dendritic cells (pDCs) via the TLR9-Myd88 pathway. To reveal the effective lactic acid bacteria (LAB) genome structure for pDCs stimulatory activity, we performed in vitro screening, using randomly selected 200 bp DNA fragments from the LC-Plasma genome. RESULTS:We found that the CpG motif copy number in the fragments was positively and significantly correlated with pDCs stimulatory activity (R = 0.491, p < 0.01). However, the determination coefficient (R2) was 0.24, which means other factors affecte activity. We found that the G + C contents of the fragment showed a significant negative correlation with activity (R = - 0.474, p < 0.01). The correlation between pDCs stimulatory activity and the copy number of CpG motifs was greatly increased when DNA fragments were stratified by G + C contents. We also performed bioinformatics analysis and a screening of LAB strains with high pDCs stimulatory activity. Species with a high copy number of CpG motifs in the low-G + C region of their genomes had higher probability of inducing high-pDCs stimulatory activity. L. lactis subsp. lactis, Leuconostoc mesenteroides, and Pediococcus pentosaceus were three typical examples of LAB that had high pDCs stimulatory activity. CONCLUSIONS:Our data suggested that the G + C content of DNA is one of the critical factors for pDCs stimulatory activity by DNA fragments. Furthermore, we found that the copy number in the low-G + C regions strongly affected the pDCs stimulatory activity of whole cells of LAB strains. These results should be useful for the design of new DNA fragments containing CpG motifs. This study also demonstrated an in silico screening method for identifying bacterial species that are able to activate pDCs. |
| 巻・号 | 19(1) |
| ページ | 88 |
| 公開日 | 2019-5-6 |
| DOI | 10.1186/s12866-019-1458-y |
| PII | 10.1186/s12866-019-1458-y |
| PMID | 31060586 |
| PMC | PMC6501324 |
| MeSH | Animals Base Composition Cells, Cultured CpG Islands DNA, Bacterial / immunology* Dendritic Cells / immunology* Female Genome, Bacterial* Genomics Lactobacillales / genetics* Mice Mice, 129 Strain Oligodeoxyribonucleotides |
| IF | 3.287 |
| 引用数 | 0 |
| リソース情報 | |
| 一般微生物 | JCM5805 JCM20101 JCM2026 JCM20109 JCM20314 JCM20459 JCM1132 JCM1551 JCM6651 JCM8341 JCM20110 |