| RRC ID |
60297
|
| 著者 |
Kominek J, Doering DT, Opulente DA, Shen XX, Zhou X, DeVirgilio J, Hulfachor AB, Groenewald M, Mcgee MA, Karlen SD, Kurtzman CP, Rokas A, Hittinger CT.
|
| タイトル |
Eukaryotic Acquisition of a Bacterial Operon.
|
| ジャーナル |
Cell
|
| Abstract |
Operons are a hallmark of bacterial genomes, where they allow concerted expression of functionally related genes as single polycistronic transcripts. They are rare in eukaryotes, where each gene usually drives expression of its own independent messenger RNAs. Here, we report the horizontal operon transfer of a siderophore biosynthesis pathway from relatives of Escherichia coli into a group of budding yeast taxa. We further show that the co-linearly arranged secondary metabolism genes are expressed, exhibit eukaryotic transcriptional features, and enable the sequestration and uptake of iron. After transfer, several genetic changes occurred during subsequent evolution, including the gain of new transcription start sites that were sometimes within protein-coding sequences, acquisition of polyadenylation sites, structural rearrangements, and integration of eukaryotic genes into the cluster. We conclude that the genes were likely acquired as a unit, modified for eukaryotic gene expression, and maintained by selection to adapt to the highly competitive, iron-limited environment.
|
| 巻・号 |
176(6)
|
| ページ |
1356-1366.e10
|
| 公開日 |
2019-3-7
|
| DOI |
10.1016/j.cell.2019.01.034
|
| PII |
S0092-8674(19)30097-2
|
| PMID |
30799038
|
| PMC |
PMC7295392
|
| MeSH |
Bacteria / genetics
Escherichia coli / genetics
Eukaryota / genetics*
Eukaryotic Cells
Evolution, Molecular
Gene Expression Regulation, Bacterial / genetics
Gene Transfer, Horizontal / genetics*
Genes, Bacterial / genetics
Genome, Bacterial / genetics
Genome, Fungal / genetics
Operon / genetics*
Saccharomycetales / genetics
Siderophores / genetics
|
| IF |
36.216
|
| 引用数 |
17
|
| リソース情報 |
| 一般微生物 |
JCM12559 |
