論文 - 詳細
| RRC ID | 603 |
|---|---|
| 著者 | Pracharoenwattana I, Cornah JE, Smith SM. |
| タイトル | Arabidopsis peroxisomal citrate synthase is required for fatty acid respiration and seed germination. |
| ジャーナル | Plant Cell |
| Abstract |
We tested the hypothesis that peroxisomal citrate synthase (CSY) is required for carbon transfer from peroxisomes to mitochondria during respiration of triacylglycerol in Arabidopsis thaliana seedlings. Two genes encoding peroxisomal CSY are expressed in Arabidopsis seedlings, and seeds from plants with both CSY genes disrupted were dormant and did not metabolize triacylglycerol. Germination was achieved by removing the seed coat and supplying sucrose, but the seedlings still did not use triacylglycerol. The mutant seedlings were resistant to 2,4-dichlorophenoxybutyric acid, indicating a block in peroxisomal beta-oxidation, and were unable to develop further after transfer to soil. The mutant phenotype was complemented with a cDNA encoding CSY with either its native peroxisomal targeting sequence (PTS2) or a heterologous PTS1 sequence from pumpkin (Cucurbita pepo) malate synthase. These results suggest that peroxisomal CSY in Arabidopsis is not only a key enzyme of the glyoxylate cycle but also catalyzes an essential step in the respiration of fatty acids. We conclude that citrate is exported from the peroxisome during fatty acid respiration, whereas in yeast, acetylcarnitine is exported. |
| 巻・号 | 17(7) |
| ページ | 2037-48 |
| 公開日 | 2005-7-1 |
| DOI | 10.1105/tpc.105.031856 |
| PII | tpc.105.031856 |
| PMID | 15923350 |
| PMC | PMC1167550 |
| MeSH | Acetylcarnitine / metabolism Arabidopsis / enzymology* Arabidopsis / genetics Arabidopsis / growth & development Cell Respiration / physiology* Citrate (si)-Synthase / genetics Citrate (si)-Synthase / metabolism* Citric Acid / metabolism DNA, Plant / genetics Fatty Acids / metabolism* Gene Expression Regulation, Enzymologic / genetics Gene Expression Regulation, Plant / genetics Germination / physiology Mitochondria / metabolism Mutation / genetics Peroxisomes / enzymology* Peroxisomes / genetics Seedlings / enzymology* Seedlings / genetics Seeds / drug effects Seeds / metabolism Sucrose / metabolism Sucrose / pharmacology Triglycerides / metabolism |
| IF | 9.618 |
| 引用数 | 141 |
| WOS 分野 | PLANT SCIENCES BIOCHEMISTRY & MOLECULAR BIOLOGY CELL BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 7 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 3.0 |
| リソース情報 | |
| シロイヌナズナ / 植物培養細胞・遺伝子 | pda02633 |