RRC ID 603
著者 Pracharoenwattana I, Cornah JE, Smith SM.
タイトル Arabidopsis peroxisomal citrate synthase is required for fatty acid respiration and seed germination.
ジャーナル Plant Cell
Abstract We tested the hypothesis that peroxisomal citrate synthase (CSY) is required for carbon transfer from peroxisomes to mitochondria during respiration of triacylglycerol in Arabidopsis thaliana seedlings. Two genes encoding peroxisomal CSY are expressed in Arabidopsis seedlings, and seeds from plants with both CSY genes disrupted were dormant and did not metabolize triacylglycerol. Germination was achieved by removing the seed coat and supplying sucrose, but the seedlings still did not use triacylglycerol. The mutant seedlings were resistant to 2,4-dichlorophenoxybutyric acid, indicating a block in peroxisomal beta-oxidation, and were unable to develop further after transfer to soil. The mutant phenotype was complemented with a cDNA encoding CSY with either its native peroxisomal targeting sequence (PTS2) or a heterologous PTS1 sequence from pumpkin (Cucurbita pepo) malate synthase. These results suggest that peroxisomal CSY in Arabidopsis is not only a key enzyme of the glyoxylate cycle but also catalyzes an essential step in the respiration of fatty acids. We conclude that citrate is exported from the peroxisome during fatty acid respiration, whereas in yeast, acetylcarnitine is exported.
巻・号 17(7)
ページ 2037-48
公開日 2005-7-1
DOI 10.1105/tpc.105.031856
PII tpc.105.031856
PMID 15923350
PMC PMC1167550
MeSH Acetylcarnitine / metabolism Arabidopsis / enzymology* Arabidopsis / genetics Arabidopsis / growth & development Cell Respiration / physiology* Citrate (si)-Synthase / genetics Citrate (si)-Synthase / metabolism* Citric Acid / metabolism DNA, Plant / genetics Fatty Acids / metabolism* Gene Expression Regulation, Enzymologic / genetics Gene Expression Regulation, Plant / genetics Germination / physiology Mitochondria / metabolism Mutation / genetics Peroxisomes / enzymology* Peroxisomes / genetics Seedlings / enzymology* Seedlings / genetics Seeds / drug effects Seeds / metabolism Sucrose / metabolism Sucrose / pharmacology Triglycerides / metabolism
IF 9.618
引用数 141
WOS 分野 PLANT SCIENCES BIOCHEMISTRY & MOLECULAR BIOLOGY CELL BIOLOGY
リソース情報
シロイヌナズナ / 植物培養細胞・遺伝子 pda02633