RRC ID 60306
著者 Rao Q, Liu Y, Chen C, Lin Q, Ren L, Huang M, Tu J, Luo T.
タイトル Pseudomonas ovata sp. nov., Isolated from the Skin of the Tail of Farmed Murray cod (Maccullochella peelii peelii) with a Profound Ulceration.
ジャーナル Curr Microbiol
Abstract A Gram-negative, strictly aerobic, motile, rod-shaped bacterium with monopolar flagellum, designated as F51T, was isolated from the skin ulcer of farmed Murray cod sampled from Zhejiang Province, China. Strain F51T grew at 4-37 °C (optimal temperature, 28 °C), pH 5.0-8.5 (optimal pH, 7.5) and NaCl concentration of 0-6.0% (w/v) (optimal concentration, 2.0%). Phylogenetic analysis based on average nucleotide identity (76.2-78.4%) and in silico DNA-DNA hybridization (22.3-23.2%) values revealed that strain F51T forms a distinct lineage in the clade of genus Pseudomonas with less than 98.9% 16S rRNA gene sequence similarity to type strains of the genus and represents a novel species related most closely to Pseudomonas floridensis LMG 30013T. Three housekeeping genes (rpoB, rpoD and gyrB) of strain F51T were analysed to further confirm that the isolate is distinctly delineated from related Pseudomonas species. Chemotaxonomic analysis indicated that the sole respiratory quinone of strain F51T is Q-9; its predominant cellular fatty acids are C16:0, summed feature 3 (iso-C15:0 2-OH and/or C16:1ω7c), summed feature 8 (C18:1ω7c and/or C18:1ω6c) and C10:0 3-OH; and its major polar lipids consist of diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, two unidentified glycolipids, three unidentified phospholipids and an unidentified aminophosphoglycolipid. This composition is typical of the chemotaxonomic attributes of Pseudomonas. Based on its phenotypic, chemotaxonomic and phylogenetic features, strain F51T is considered to represent a novel species for which the name Pseudomonas ovata sp. nov. is proposed. The type strain is F51T (= KCTC 62133T = MCCC 1K03458T).
巻・号 76(10)
ページ 1168-1174
公開日 2019-10-1
DOI 10.1007/s00284-019-01729-1
PII 10.1007/s00284-019-01729-1
PMID 31263923
MeSH Animals Aquaculture Base Composition China DNA, Bacterial / genetics Fish Diseases / microbiology* Genes, Bacterial / genetics Genome, Bacterial / genetics Lipids / chemistry Nucleic Acid Hybridization Perciformes / microbiology* Phylogeny* Pseudomonas / chemistry Pseudomonas / classification* Pseudomonas / genetics Pseudomonas / physiology* RNA, Ribosomal, 16S / genetics Sequence Analysis, DNA Species Specificity
IF 1.595
引用数 1
リソース情報
一般微生物 JCM5962