RRC ID 60573
著者 Sato S, Fukuoka T, Saika A, Koshiyama T, Morita T.
タイトル A New Screening Approach for Glycolipid-type Biosurfactant Producers Using MALDI-TOF/MS.
ジャーナル J Oleo Sci
Abstract We applied matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF/MS) to screen for glycolipid-type biosurfactant (BS) producers. A crude extract of Pseudozyma antarctica, a well-known mannosylerythritol lipid (MEL) producer, was initially subjected to MALDI-TOF/MS. The spectrum of the extract showed the accumulation of diacylated MELs in culture. We then screened 80 environmental samples for BS-producing yeasts, and extracts from broth cultures of the selected five strains were examined using MALDI-TOF/MS. The results showed that all five strains produced MELs, whereas four strains also produced cellobiose lipids (CLs). By D1/D2 region sequence analysis, the MEL-producing strain was assigned to P. antarctica while the four MEL- and CL-producing strains were assigned to P. hubeiensis. These results demonstrate that MALDI-TOF/MS is a rapid and reliable tool to detect BS molecules in crude extracts of broth cultures to screen for glycolipid-type BS producers.
巻・号 68(12)
ページ 1287-1294
公開日 2019-1-1
DOI 10.5650/jos.ess19177
PMID 31787677
MeSH Glycolipids / analysis* Phylogeny Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods Surface-Active Agents / analysis* Ustilaginales / chemistry Ustilaginales / classification Ustilaginales / genetics
IF 1.304
引用数 0
リソース情報
一般微生物 JCM10317