論文 - 詳細
| RRC ID | 60613 |
|---|---|
| 著者 | Watanabe S, Fukumori F, Watanabe Y. |
| タイトル | Substrate and metabolic promiscuities of d-altronate dehydratase family proteins involved in non-phosphorylative d-arabinose, sugar acid, l-galactose and l-fucose pathways from bacteria. |
| ジャーナル | Mol Microbiol |
| Abstract |
The gene context in microorganism genomes is of considerable help for identifying potential substrates. The C785_RS13685 gene in Herbaspirillum huttiense IAM 15032 is a member of the d-altronate dehydratase protein family, and which functions as a d-arabinonate dehydratase in vitro, is clustered with genes related to putative pentose metabolism. In the present study, further biochemical characterization and gene expression analyses revealed that l-xylonate is a physiological substrate that is ultimately converted to α-ketoglutarate via so-called Route II of a non-phosphorylative pathway. Several hexonates, including d-altronate, d-idonate and l-gluconate, which are also substrates of C785_RS13685, also significantly up-regulated the gene cluster containing C785_RS13685, suggesting a possibility that pyruvate and d- or l-glycerate were ultimately produced (novel Route III). On the contrary, ACAV_RS08155 of Acidovorax avenae ATCC 19860, a homologous gene to C785_RS13685, functioned as a d-altronate dehydratase in a novel l-galactose pathway, through which l-galactonate was epimerized at the C5 position by the sequential activity of two dehydrogenases, resulting in d-altronate. Furthermore, this pathway completely overlapped with Route III of the non-phosphorylative l-fucose pathway. The 'substrate promiscuity' of d-altronate dehydratase protein(s) is significantly expanded to 'metabolic promiscuity' in the d-arabinose, sugar acid, l-fucose and l-galactose pathways. |
| 巻・号 | 112(1) |
| ページ | 147-165 |
| 公開日 | 2019-7-1 |
| DOI | 10.1111/mmi.14259 |
| PMID | 30985034 |
| MeSH | Amino Acid Sequence / genetics Arabinose / metabolism Cloning, Molecular / methods Fucose / metabolism Galactose / metabolism Genome, Bacterial / genetics Gluconates / metabolism Herbaspirillum / genetics Herbaspirillum / metabolism Hydro-Lyases / genetics* Hydro-Lyases / metabolism* Hydro-Lyases / physiology Multigene Family / genetics Sugar Acids / metabolism |
| IF | 3.649 |
| 引用数 | 2 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 2 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 一般微生物 | JCM20985 |