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  • Search Condition : Filter (MeSH = Cloning, Molecular / methods)
Species Resource
Prokaryotes E. coli CV-25(DH5alpha) , pINT-ts , pAH69 , pAH121 , pAH130 Replicon-free and markerless methods for genomic insertion of DNAs in phage attachment sites and controlled expression of chromosomal genes in Escherichia coli.
Zebrafish rw0 Hedgehog and Fgf signaling pathways regulate the development of tphR-expressing serotonergic raphe neurons in zebrafish embryos.
Yeast FY7266 , FY7269 , FY7519 , FY12697 Identification and functional analysis of the erh1(+) gene encoding enhancer of rudimentary homolog from the fission yeast Schizosaccharomyces pombe.
Prokaryotes E. coli ME5076 Use of bacteriophage lambda recombination functions to promote gene replacement in Escherichia coli.
Prokaryotes E. coli pHA10 Plasmid vectors for positive selection of DNA inserts controlled by the lambda pL promoter, repressor and antitermination function.
Prokaryotes E. coli pBT7 A bacteriophage T7-based expression vector, pBT7, with color selection for the recombinant.
Prokaryotes E. coli pETHis , pETHisK Expression vectors for affinity purification and radiolabeling of proteins using Escherichia coli as host.
Prokaryotes E. coli pKF4 Detection of exonuclease activities in restriction endonuclease preparations using an enforcement plasmid for kanamycin-resistance selection.
Prokaryotes E. coli pMT440 A plasmid vector with positive selection and directional cloning based on a conditionally lethal gene.
Prokaryotes E. coli pEMBL18- , pEMBL18+ , pEMBL19- , pEMBL19+ pEMBL: a new family of single-stranded plasmids for sequencing DNA.
C.elegans tm2484 A seven-transmembrane receptor that mediates avoidance response to dihydrocaffeic acid, a water-soluble repellent in Caenorhabditis elegans.
Prokaryotes E. coli pKF16c , CV-55(Om279) A novel dual vector coexpressing PhiX174 lysis E gene and staphylococcal nuclease A gene on the basis of lambda promoter pR and pL, respectively.
DNA material PCCS7 (RDB01538) Molecular cloning of a cysteine synthase cDNA from Citrullus vulgaris (watermelon) by genetic complementation in an Escherichia coli Cys- auxotroph.
Silkworms n17 Expression and Characterization of Human β-1, 4-Galactosyltransferase 1 (β4GalT1) Using Silkworm-Baculovirus Expression System.
DNA material prNTR 2 ( full8-6 ) (RDB13190) Structure and functional expression of the cloned rat neurotensin receptor.
Pathogenic microorganisms NA Positional cloning in Cryptococcus neoformans and its application for identification and cloning of the gene encoding methylenetetrahydrofolate reductase.
Zebrafish ? In vivo somatic delivery of plasmid DNA and retrograde transport to obtain cell-specific gene expression in the central nervous system.
Prokaryotes E. coli pHRP308 Construction and characterization of thermo-inducible vectors derived from heat-sensitive lacI genes in combination with the T7 A1 promoter.
General Microbes JCM 9174 Gene cloning and characterization of dihydrolipoamide dehydrogenase from Microbacterium luteolum: A useful enzymatic regeneration system of NAD+ from NADH.
General Microbes JCM 7364 , JCM 7686 DIY series of genetic cassettes useful in construction of versatile vectors specific for Alphaproteobacteria.