| RRC ID |
60616
|
| 著者 |
Forster SC, Kumar N, Anonye BO, Almeida A, Viciani E, Stares MD, Dunn M, Mkandawire TT, Zhu A, Shao Y, Pike LJ, Louie T, Browne HP, Mitchell AL, Neville BA, Finn RD, Lawley TD.
|
| タイトル |
A human gut bacterial genome and culture collection for improved metagenomic analyses.
|
| ジャーナル |
Nat Biotechnol
|
| Abstract |
Understanding gut microbiome functions requires cultivated bacteria for experimental validation and reference bacterial genome sequences to interpret metagenome datasets and guide functional analyses. We present the Human Gastrointestinal Bacteria Culture Collection (HBC), a comprehensive set of 737 whole-genome-sequenced bacterial isolates, representing 273 species (105 novel species) from 31 families found in the human gastrointestinal microbiota. The HBC increases the number of bacterial genomes derived from human gastrointestinal microbiota by 37%. The resulting global Human Gastrointestinal Bacteria Genome Collection (HGG) classifies 83% of genera by abundance across 13,490 shotgun-sequenced metagenomic samples, improves taxonomic classification by 61% compared to the Human Microbiome Project (HMP) genome collection and achieves subspecies-level classification for almost 50% of sequences. The improved resource of gastrointestinal bacterial reference sequences circumvents dependence on de novo assembly of metagenomes and enables accurate and cost-effective shotgun metagenomic analyses of human gastrointestinal microbiota.
|
| 巻・号 |
37(2)
|
| ページ |
186-192
|
| 公開日 |
2019-2-1
|
| DOI |
10.1038/s41587-018-0009-7
|
| PII |
10.1038/s41587-018-0009-7
|
| PMID |
30718869
|
| PMC |
PMC6785715
|
| MeSH |
Bacteria / classification
Computational Biology / methods
Contig Mapping
Gastrointestinal Microbiome
Genome, Bacterial*
Genome, Human
Humans
Metagenome*
Metagenomics*
Phylogeny
RNA, Ribosomal, 16S / metabolism
Sequence Analysis, DNA
Species Specificity
|
| IF |
31.864
|
| 引用数 |
51
|
| リソース情報 |
| 一般微生物 |
JCM31252
JCM31253
JCM31267
JCM31265
JCM31268
JCM31257
JCM31261
JCM31262
JCM31260
JCM31255 |
