| RRC ID |
60621
|
| 著者 |
Zhang L, Yuan L, Staehelin C, Li Y, Ruan J, Liang Z, Xie Z, Wang W, Xie J, Huang S.
|
| タイトル |
The LYSIN MOTIF-CONTAINING RECEPTOR-LIKE KINASE 1 protein of banana is required for perception of pathogenic and symbiotic signals.
|
| ジャーナル |
New Phytol
|
| Abstract |
How plants can distinguish pathogenic and symbiotic fungi remains largely unknown. Here, we characterized the role of MaLYK1, a lysin motif receptor kinase of banana. Live cell imaging techniques were used in localization studies. RNA interference (RNAi)-silenced transgenic banana plants were generated to analyze the biological role of MaLYK1. The MaLYK1 ectodomain, chitin beads, chitooligosaccharides (COs) and mycorrhizal lipochitooligosaccharides (Myc-LCOs) were used in pulldown assays. Ligand-induced MaLYK1 complex formation was tested in immunoprecipitation experiments. Chimeric receptors were expressed in Lotus japonicus to characterize the function of the MaLYK1 kinase domain. MaLYK1 was localized to the plasma membrane. MaLYK1 expression was induced by Foc4 (Fusarium oxysporum f. sp. cubense race 4) and diverse microbe-associated molecular patterns. MaLYK1-silenced banana lines showed reduced chitin-triggered defense responses, increased Foc4-induced disease symptoms and reduced mycorrhization. The MaLYK1 ectodomain was pulled down by chitin beads and LCOs or COs impaired this process. Ligand treatments induced MaLYK1 complex formation in planta. The kinase domain of MaLYK1 could functionally replace that of the chitin elicitor receptor kinase 1 (AtCERK1) in Arabidopsis thaliana and of a rhizobial LCO (Nod factor) receptor (LjNFR1) in L. japonicus. MaLYK1 represents a central molecular switch that controls defense- and symbiosis-related signaling.
|
| 巻・号 |
223(3)
|
| ページ |
1530-1546
|
| 公開日 |
2019-8-1
|
| DOI |
10.1111/nph.15888
|
| PMID |
31059122
|
| MeSH |
Arabidopsis / metabolism
Chitin / analogs & derivatives
Chitin / metabolism
Chitosan
Gene Expression Regulation, Plant
Gene Silencing
Lotus / metabolism
Musa / genetics
Musa / metabolism*
Musa / microbiology*
Mycorrhizae / physiology
Oligosaccharides
Pathogen-Associated Molecular Pattern Molecules / metabolism
Plant Proteins / chemistry
Plant Proteins / metabolism*
Protein Domains
RNA, Messenger / genetics
RNA, Messenger / metabolism
Signal Transduction*
Symbiosis*
|
| IF |
7.299
|
| 引用数 |
2
|
| リソース情報 |
| 一般微生物 |
JCM21590 |
