RRC ID 60621
Author Zhang L, Yuan L, Staehelin C, Li Y, Ruan J, Liang Z, Xie Z, Wang W, Xie J, Huang S.
Title The LYSIN MOTIF-CONTAINING RECEPTOR-LIKE KINASE 1 protein of banana is required for perception of pathogenic and symbiotic signals.
Journal New Phytol.
Abstract How plants can distinguish pathogenic and symbiotic fungi remains largely unknown. Here, we characterized the role of MaLYK1, a lysin motif receptor kinase of banana. Live cell imaging techniques were used in localization studies. RNA interference (RNAi)-silenced transgenic banana plants were generated to analyze the biological role of MaLYK1. The MaLYK1 ectodomain, chitin beads, chitooligosaccharides (COs) and mycorrhizal lipochitooligosaccharides (Myc-LCOs) were used in pulldown assays. Ligand-induced MaLYK1 complex formation was tested in immunoprecipitation experiments. Chimeric receptors were expressed in Lotus japonicus to characterize the function of the MaLYK1 kinase domain. MaLYK1 was localized to the plasma membrane. MaLYK1 expression was induced by Foc4 (Fusarium oxysporum f. sp. cubense race 4) and diverse microbe-associated molecular patterns. MaLYK1-silenced banana lines showed reduced chitin-triggered defense responses, increased Foc4-induced disease symptoms and reduced mycorrhization. The MaLYK1 ectodomain was pulled down by chitin beads and LCOs or COs impaired this process. Ligand treatments induced MaLYK1 complex formation in planta. The kinase domain of MaLYK1 could functionally replace that of the chitin elicitor receptor kinase 1 (AtCERK1) in Arabidopsis thaliana and of a rhizobial LCO (Nod factor) receptor (LjNFR1) in L. japonicus. MaLYK1 represents a central molecular switch that controls defense- and symbiosis-related signaling.
Volume 223(3)
Pages 1530-1546
Published 2019-8
DOI 10.1111/nph.15888
PMID 31059122
MeSH Arabidopsis / metabolism Chitin / analogs & derivatives Chitin / metabolism Gene Expression Regulation, Plant Gene Silencing Lotus / metabolism Musa / genetics Musa / metabolism* Musa / microbiology* Mycorrhizae / physiology Pathogen-Associated Molecular Pattern Molecules / metabolism Plant Proteins / chemistry Plant Proteins / metabolism* Protein Domains RNA, Messenger / genetics RNA, Messenger / metabolism Signal Transduction* Symbiosis*
IF 7.299
Times Cited 2
Resource
General Microbes JCM21590