RRC ID 60622
著者 Pareek S, Kurakawa T, Das B, Motooka D, Nakaya S, Rongsen-Chandola T, Goyal N, Kayama H, Dodd D, Okumura R, Maeda Y, Fujimoto K, Nii T, Ogawa T, Iida T, Bhandari N, Kida T, Nakamura S, Nair GB, Takeda K.
タイトル Comparison of Japanese and Indian intestinal microbiota shows diet-dependent interaction between bacteria and fungi.
ジャーナル NPJ Biofilms Microbiomes
Abstract The bacterial species living in the gut mediate many aspects of biological processes such as nutrition and activation of adaptive immunity. In addition, commensal fungi residing in the intestine also influence host health. Although the interaction of bacterium and fungus has been shown, its precise mechanism during colonization of the human intestine remains largely unknown. Here, we show interaction between bacterial and fungal species for utilization of dietary components driving their efficient growth in the intestine. Next generation sequencing of fecal samples from Japanese and Indian adults revealed differential patterns of bacterial and fungal composition. In particular, Indians, who consume more plant polysaccharides than Japanese, harbored increased numbers of Prevotella and Candida. Candida spp. showed strong growth responses to the plant polysaccharide arabinoxylan in vitro. Furthermore, the culture supernatants of Candida spp. grown with arabinoxylan promoted rapid proliferation of Prevotella copri. Arabinose was identified as a potential growth-inducing factor in the Candida culture supernatants. Candida spp. exhibited a growth response to xylose, but not to arabinose, whereas P. copri proliferated in response to both xylose and arabinose. Candida spp., but not P. copri, colonized the intestine of germ-free mice. However, P. copri successfully colonized mouse intestine already harboring Candida. These findings demonstrate a proof of concept that fungal members of gut microbiota can facilitate a colonization of the intestine by their bacterial counterparts, potentially mediated by a dietary metabolite.
巻・号 5(1)
ページ 37
公開日 2019-1-1
DOI 10.1038/s41522-019-0110-9
PII 110
PMID 31885873
PMC PMC6925221
MeSH Animals Bacteria / classification Bacteria / growth & development* Bacteria / metabolism* Diet / methods* Feces / microbiology Fungi / classification Fungi / growth & development* Fungi / metabolism* Gastrointestinal Microbiome* Humans India Japan Mice Microbial Interactions* Models, Animal Polysaccharides / metabolism
IF 7.067
引用数 3
リソース情報
一般微生物 JCM13464 JCM11019 JCM5824 JCM5827 JCM5828 JCM1541 JCM1542 JCM7255