| RRC ID |
60641
|
| 著者 |
Chaudhary DK, Kim DU, Kim D, Kim J.
|
| タイトル |
Flavobacterium petrolei sp. nov., a novel psychrophilic, diesel-degrading bacterium isolated from oil-contaminated Arctic soil.
|
| ジャーナル |
Sci Rep
|
| Abstract |
This study presents taxonomic description of two novel diesel-degrading, psychrophilic strains: Kopri-42T and Kopri-43, isolated during screening of oil-degrading psychrotrophs from oil-contaminated Arctic soil. A preliminary 16S rRNA gene sequence and phylogenetic tree analysis indicated that these Arctic strains belonged to the genus Flavobacterium, with the nearest relative being Flavobacterium psychrolimnae LMG 22018T (98.9% sequence similarity). The pairwise 16S rRNA gene sequence identity between strains Kopri-42T and Kopri-43 was 99.7%. The DNA-DNA hybridization value between strain Kopri-42T and Kopri-43 was 88.6 ± 2.1% indicating that Kopri-42T and Kopri-43 represents two strains of the same genomospecies. The average nucleotide identity and in silico DNA-DNA hybridization values between strain Kopri-42T and nearest relative F. psychrolimnae LMG 22018T were 92.4% and 47.9%, respectively. These values support the authenticity of the novel species and confirmed the strain Kopri-42T belonged to the genus Flavobacterium as a new member. The morphological, physiological, biochemical and chemotaxonomic data also distinguished strain Kopri-42T from its closest phylogenetic neighbors. Based on the polyphasic data, strains Kopri-42T and Kopri-43 represents a single novel species of the genus Flavobacterium, for which the name Flavobacterium petrolei sp. nov. is proposed. The type strain is Kopri-42T (=KEMB 9005-710T = KACC 19625T = NBRC 113374T).
|
| 巻・号 |
9(1)
|
| ページ |
4134
|
| 公開日 |
2019-3-11
|
| DOI |
10.1038/s41598-019-40667-7
|
| PII |
10.1038/s41598-019-40667-7
|
| PMID |
30858439
|
| PMC |
PMC6411956
|
| MeSH |
Arctic Regions
Biotransformation
Flavobacterium / classification
Flavobacterium / genetics*
Flavobacterium / metabolism
Gasoline / microbiology*
Genome, Bacterial
Phylogeny*
Sequence Homology, Nucleic Acid
Soil Microbiology
Soil Pollutants / metabolism*
|
| IF |
4.011
|
| 引用数 |
4
|
| リソース情報 |
| 一般微生物 |
JCM16398
JCM15087 |
