| RRC ID |
60657
|
| 著者 |
Menon RR, Kumari S, Kumar P, Verma A, Krishnamurthi S, Rameshkumar N.
|
| タイトル |
Sphingomonas pokkalii sp. nov., a novel plant associated rhizobacterium isolated from a saline tolerant pokkali rice and its draft genome analysis.
|
| ジャーナル |
Syst Appl Microbiol
|
| Abstract |
Three strains L3B27T, 3CNBAF, L1A4 isolated from a brackish cultivated pokkali rice rhizosphere were characterised using a polyphasic taxonomic approach. Phylogenetic analysis based on 16S rRNA and recA gene sequences revealed that these strains were highly similar among each other and formed a separate monophyletic cluster within the genus Sphingomonas with Sphingomonas pituitosa DSM 13101T, Sphingomonas azotifigens DSM 18530T and Sphingomonas trueperi DSM 7225T as their closest relatives sharing 97.9-98.3% 16S rRNA similarity and 91.3-94.0% recA similarity values, respectively. The average nucleotide identity (ANI), average amino acid identity (AAI) and digital DNA-DNA hybridisation (dDDH) values between L3B27T (representative of the novel strains) and its phylogenetically closest Sphingomonas species were well below the established cut-off <94% (ANI/AAI) and <70% (dDDH) for species delineation. Further, the novel strains can be distinguished from its closest relatives based on several phenotypic traits. Thus, based on the polyphasic approach, we describe a novel Sphingomonas species for which the name Sphingomonas pokkalii sp. nov (type strain L3B27T=KCTC 42098T=MCC 3001T) is proposed. In addition, the novel strains were characterised for their plant associated properties and found to possess several phenotypic traits which probably explain its plant associated lifestyle. This was further confirmed by the presence of several plant associated gene features in the genome of L3B27T. Also, we could identify gene features which may likely involve in brackish water adaptation. Thus, this study provides first insights into the plant associated lifestyle, genome and taxonomy of a novel brackish adapted plant associated Sphingomonas.
|
| 巻・号 |
42(3)
|
| ページ |
334-342
|
| 公開日 |
2019-5-1
|
| DOI |
10.1016/j.syapm.2019.02.003
|
| PII |
S0723-2020(18)30448-X
|
| PMID |
30808585
|
| MeSH |
DNA, Bacterial / genetics
Fatty Acids / analysis
Genes, Bacterial / genetics
Genome, Bacterial / genetics*
Lipids / analysis
Nucleic Acid Hybridization
Oryza / microbiology*
Oryza / physiology
Phylogeny*
Polyamines / analysis
Quinones / analysis
RNA, Ribosomal, 16S / genetics
Rhizosphere*
Salt Tolerance
Sequence Analysis, DNA
Soil Microbiology
Sphingomonas / chemistry
Sphingomonas / classification*
Sphingomonas / genetics*
|
| IF |
2.808
|
| 引用数 |
0
|
| リソース情報 |
| 一般微生物 |
JCM18825 |
