論文 - 詳細
| RRC ID | 60682 |
|---|---|
| 著者 | Molinier J, Lechner E, Dumbliauskas E, Genschik P. |
| タイトル | Regulation and role of Arabidopsis CUL4-DDB1A-DDB2 in maintaining genome integrity upon UV stress. |
| ジャーナル | PLoS Genet |
| Abstract |
Plants use the energy in sunlight for photosynthesis, but as a consequence are exposed to the toxic effect of UV radiation especially on DNA. The UV-induced lesions on DNA affect both transcription and replication and can also have mutagenic consequences. Here we investigated the regulation and the function of the recently described CUL4-DDB1-DDB2 E3 ligase in the maintenance of genome integrity upon UV-stress using the model plant Arabidopsis. Physiological, biochemical, and genetic evidences indicate that this protein complex is involved in global genome repair (GGR) of UV-induced DNA lesions. Moreover, we provide evidences for crosstalks between GGR, the plant-specific photo reactivation pathway and the RAD1-RAD10 endonucleases upon UV exposure. Finally, we report that DDB2 degradation upon UV stress depends not only on CUL4, but also on the checkpoint protein kinase Ataxia telangiectasia and Rad3-related (ATR). Interestingly, we found that DDB1A shuttles from the cytoplasm to the nucleus in an ATR-dependent manner, highlighting an upstream level of control and a novel mechanism of regulation of this E3 ligase. |
| 巻・号 | 4(6) |
| ページ | e1000093 |
| 公開日 | 2008-6-13 |
| DOI | 10.1371/journal.pgen.1000093 |
| PMID | 18551167 |
| PMC | PMC2396500 |
| MeSH | Arabidopsis / genetics Arabidopsis / growth & development Arabidopsis / metabolism Arabidopsis / radiation effects* Arabidopsis Proteins / analysis Arabidopsis Proteins / genetics Arabidopsis Proteins / metabolism* Ataxia Telangiectasia Mutated Proteins Calcium-Binding Proteins / genetics Calcium-Binding Proteins / metabolism Cell Cycle Proteins / metabolism Cullin Proteins / analysis Cullin Proteins / genetics Cullin Proteins / metabolism* DNA Damage / radiation effects DNA Repair / radiation effects DNA-Binding Proteins / analysis DNA-Binding Proteins / genetics DNA-Binding Proteins / metabolism* Gene Expression Regulation, Plant / radiation effects Genome, Plant / radiation effects* Mutagenesis, Insertional Plants, Genetically Modified / genetics Plants, Genetically Modified / growth & development Plants, Genetically Modified / metabolism Plants, Genetically Modified / radiation effects Protein Serine-Threonine Kinases / metabolism Protein Transport Radiation Tolerance Transcription Factors / genetics Transcription Factors / metabolism Ultraviolet Rays* |
| IF | 5.224 |
| 引用数 | 75 |
| リソース情報 | |
| シロイヌナズナ / 植物培養細胞・遺伝子 | pst00853 |