Reference - Detail
|Author||Molinier J, Lechner E, Dumbliauskas E, Genschik P.|
|Title||Regulation and role of Arabidopsis CUL4-DDB1A-DDB2 in maintaining genome integrity upon UV stress.|
Plants use the energy in sunlight for photosynthesis, but as a consequence are exposed to the toxic effect of UV radiation especially on DNA. The UV-induced lesions on DNA affect both transcription and replication and can also have mutagenic consequences. Here we investigated the regulation and the function of the recently described CUL4-DDB1-DDB2 E3 ligase in the maintenance of genome integrity upon UV-stress using the model plant Arabidopsis. Physiological, biochemical, and genetic evidences indicate that this protein complex is involved in global genome repair (GGR) of UV-induced DNA lesions. Moreover, we provide evidences for crosstalks between GGR, the plant-specific photo reactivation pathway and the RAD1-RAD10 endonucleases upon UV exposure. Finally, we report that DDB2 degradation upon UV stress depends not only on CUL4, but also on the checkpoint protein kinase Ataxia telangiectasia and Rad3-related (ATR). Interestingly, we found that DDB1A shuttles from the cytoplasm to the nucleus in an ATR-dependent manner, highlighting an upstream level of control and a novel mechanism of regulation of this E3 ligase.
|MeSH||Arabidopsis / genetics Arabidopsis / growth & development Arabidopsis / metabolism Arabidopsis / radiation effects* Arabidopsis Proteins / analysis Arabidopsis Proteins / genetics Arabidopsis Proteins / metabolism* Ataxia Telangiectasia Mutated Proteins Calcium-Binding Proteins / genetics Calcium-Binding Proteins / metabolism Cell Cycle Proteins / metabolism Cullin Proteins / analysis Cullin Proteins / genetics Cullin Proteins / metabolism* DNA Damage / radiation effects DNA Repair / radiation effects DNA-Binding Proteins / analysis DNA-Binding Proteins / genetics DNA-Binding Proteins / metabolism* Gene Expression Regulation, Plant / radiation effects Genome, Plant / radiation effects* Mutagenesis, Insertional Plants, Genetically Modified / genetics Plants, Genetically Modified / growth & development Plants, Genetically Modified / metabolism Plants, Genetically Modified / radiation effects Protein Transport Protein-Serine-Threonine Kinases / metabolism Radiation Tolerance Transcription Factors / genetics Transcription Factors / metabolism Ultraviolet Rays*|
|Arabidopsis / Cultured plant cells, genes||pst00853|