| RRC ID |
60685
|
| 著者 |
Okuzaki Y, Kaneoka H, Suzuki T, Hagihara Y, Nakayama Y, Murakami S, Murase Y, Kuroiwa A, Iijima S, Nishijima KI.
|
| タイトル |
PRDM14 and BLIMP1 control the development of chicken primordial germ cells.
|
| ジャーナル |
Dev Biol
|
| Abstract |
The differentiation of primordial germ cells (PGCs) is a fundamental step in development. PR domain-containing protein 14 (PRDM14) and B lymphocyte-induced maturation protein 1 (BLIMP1) play pivotal roles in mouse PGC specification. In the present study, we assessed the roles of chicken orthologs of PRDM14 and BLIMP1 in PGC development. PRDM14 and BLIMP1 were expressed in blastodermal cells and PGCs. The in vivo knockdown of PRDM14 or BLIMP1 by introducing a replication-competent retroviral vector expressing shRNAs to the blastodermal stage of embryos reduced the number of SSEA-1 or chicken vasa homologue-positive PGCs on day 5.5-6.5. Since the inhibition of Activin receptor-like kinase 4/5/7 in cultured PGCs reduced the expression of PRDM14, BLIMP1, and NANOG, and that of MEK inhibited PRDM14 expression, the expression of these genes seems to be controlled by Activin A and FGF2 signaling. Overall, PRDM14, BLIMP1, and NANOG seem to be involved in the self-renewal of PGCs in cultured PGCs and embryos.
|
| 巻・号 |
455(1)
|
| ページ |
32-41
|
| 公開日 |
2019-11-1
|
| DOI |
10.1016/j.ydbio.2019.06.018
|
| PII |
S0012-1606(18)30799-1
|
| PMID |
31271752
|
| MeSH |
Animals
Avian Proteins / genetics*
Avian Proteins / metabolism
Blastoderm / cytology
Blastoderm / metabolism
Cell Differentiation / genetics*
Cell Self Renewal / genetics
Cells, Cultured
Chick Embryo
Chickens
Gene Expression Regulation, Developmental*
Germ Cells / cytology
Germ Cells / metabolism*
Lewis X Antigen / genetics
Lewis X Antigen / metabolism
Nanog Homeobox Protein / genetics
Nanog Homeobox Protein / metabolism
Positive Regulatory Domain I-Binding Factor 1 / genetics*
Positive Regulatory Domain I-Binding Factor 1 / metabolism
RNA Interference
|
| IF |
2.936
|
| 引用数 |
1
|
| リソース情報 |
| ニワトリ・ウズラ |
White Leghorn |
