論文 - 詳細
| RRC ID | 60714 |
|---|---|
| 著者 | Hwang JH, Vuong LT, Choi KW. |
| タイトル | Crumbs, Galla and Xpd are required for Kinesin-5 regulation in mitosis and organ growth in Drosophila. |
| ジャーナル | J Cell Sci |
| Abstract |
Xeroderma Pigmentosum D (XPD, also known as ERCC2) is a multi-functional protein involved in transcription, DNA repair and chromosome segregation. In Drosophila, Xpd interacts with Crumbs (Crb) and Galla to regulate mitosis during embryogenesis. It is unknown how these proteins are linked to mitosis. Here, we show that Crb, Galla-2 and Xpd regulate nuclear division in the syncytial embryo by interacting with Klp61F, the Drosophila mitotic Kinesin-5 associated with bipolar spindles. Crb, Galla-2 and Xpd physically interact with Klp61F and colocalize to mitotic spindles. Knockdown of any of these proteins results in similar mitotic defects. These phenotypes are restored by overexpression of Klp61F, suggesting that Klp61F is a major effector. Mitotic defects of galla-2 RNAi are suppressed by Xpd overexpression but not vice versa. Depletion of Crb, Galla-2 or Xpd results in a reduction of Klp61F levels. Reducing proteasome function restores Klp61F levels and suppresses mitotic defects caused by knockdown of Crb, Galla-2 or Xpd. Furthermore, eye growth is regulated by Xpd and Klp61F. Hence, we propose that Crb, Galla-2 and Xpd interact to maintain the level of Klp61F during mitosis and organ growth. |
| 巻・号 | 133(12) |
| 公開日 | 2020-6-23 |
| DOI | 10.1242/jcs.246801 |
| PII | jcs.246801 |
| PMID | 32501288 |
| MeSH | Animals Drosophila* Drosophila Proteins* / genetics Kinesins / genetics Microtubule-Associated Proteins / genetics Mitosis |
| IF | 4.573 |
| 引用数 | 0 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 3 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ショウジョウバエ | |