RRC ID 6078
Author Kim YW, Kato K, Hirai T, Hiwasa-Tanase K, Ezura H.
Title Spatial and developmental profiling of miraculin accumulation in transgenic tomato fruits expressing the miraculin gene constitutively.
Journal J Agric Food Chem
Abstract We previously developed a transgenic tomato that expresses the miraculin gene using a constitutive promoter. In this study, we profiled the developmental and spatial accumulation of the miraculin protein and mRNA in transgenic tomato fruits. Miraculin mRNA expression was almost constant up to orange stage, and then the expression increased at red stage. The miraculin protein accumulated gradually during fruit development and reached its highest level at the overripe stage. At the red stage of fruit, miraculin protein was accumulated at the highest level in the exocarp, and similar in other fruit tissues: mesocarp, dissepiment, upper placenta, lower placenta and jelly. Moreover, the pattern of miraculin accumulation in fruit tissues was the same regardless of genetic background and position at which the miraculin gene was inserted in the genome. We also discuss suitable tomato types expressing miraculin for their commercial use.
Volume 58(1)
Pages 282-6
Published 2010-1-13
DOI 10.1021/jf9030663
PMID 20014854
MeSH Fruit / genetics Fruit / growth & development Fruit / metabolism Gene Expression Regulation, Developmental* Lycopersicon esculentum / genetics Lycopersicon esculentum / growth & development Lycopersicon esculentum / metabolism* Plant Proteins / genetics* Plant Proteins / metabolism* Plants, Genetically Modified / genetics Plants, Genetically Modified / growth & development Plants, Genetically Modified / metabolism*
IF 4.192
Times Cited 22
Tomato TOMJPF00001 TOMJPF00002(Moneymaker)