論文 - 詳細
| RRC ID | 60836 |
|---|---|
| 著者 | Min C, Park J, Kim G, Moon H, Lee SA, Kim D, Moon B, Yang S, Lee J, Kim K, Cho H, Park J, Lee DH, Lee G, Park D. |
| タイトル | Tim-4 functions as a scavenger receptor for phagocytosis of exogenous particles. |
| ジャーナル | Cell Death Dis |
| Abstract |
The phosphatidylserine (PS) receptor Tim-4 mediates phagocytosis of apoptotic cells by binding to PS exposed on the surface of these cells, and thus functions as a PS receptor for apoptotic cells. Some of PS receptors are capable of recognizing other molecules, such as LPS on bacteria, besides PS on apoptotic cells. However, it is unclear whether Tim-4 perceives other molecules like the PS receptors. Here, we report that Tim-4 facilitates the phagocytosis of exogenous particles as well as apoptotic cells. Similar to the process that occurs during Tim-4-mediated efferocytosis, the uptake of exogenous E. coli and S. aureus bioparticles was promoted by overexpression of Tim-4 on phagocytes, whereas phagocytosis of the bioparticles was reduced in Tim-4-deficient cells. A truncation mutant of Tim-4 lacking the cytoplasmic tail promoted phagocytosis of the particles, but a mutant lacking the IgV or the mucin domain failed to enhance phagocytosis. However, expression of Tim-4AAA (a mutant form of Tim-4 that does not bind phosphatidylserine and does not promote efferocytosis) still promoted phagocytosis. Tim-4-mediated phagocytosis was not blocked by expression of the phosphatidylserine-binding protein Anxa5. Furthermore, binding of lipopolysaccharide (LPS), which is found in the outer membrane of Gram-negative bacteria, was higher in Tim-4-overexpressing cells than in Tim-4-deficient cells. In summary, our study suggests that Tim-4 acts as a scavenger receptor and mediates phagocytosis of exogenous particles in a phosphatidylserine-independent manner. |
| 巻・号 | 11(7) |
| ページ | 561 |
| 公開日 | 2020-7-23 |
| DOI | 10.1038/s41419-020-02773-7 |
| PII | 10.1038/s41419-020-02773-7 |
| PMID | 32703939 |
| PMC | PMC7378189 |
| MeSH | Animals Apoptosis Cell Line Escherichia coli / metabolism Membrane Proteins / chemistry Membrane Proteins / metabolism* Mice, Inbred C57BL Phagocytes / metabolism Phagocytosis* Phosphatidylserines / metabolism Receptors, Scavenger / metabolism* Staphylococcus aureus / metabolism |
| IF | 5.959 |
| 引用数 | 0 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 実験動物マウス | RBRC04885 |