論文 - 詳細
| RRC ID | 60837 |
|---|---|
| 著者 | Yang DW, Choi KW. |
| タイトル | Suppression of Patronin deficiency by altered Hippo signaling in Drosophila organ development. |
| ジャーナル | Cell Death Differ |
| Abstract |
The microtubule network is crucial for cell structure and function. Patronin is a conserved protein involved in protecting the minus end of microtubules. Conversely, Klp10A is a kinesin-like microtubule depolymerase. Here we report the role of Drosophila Patronin and Klp10A for cell survival in developing organs. Loss of Patronin reduces the size of organs by activation of a caspase in imaginal discs. Reduced wing by Patronin RNAi is suppressed by knockdown of Spastin (Spas) but not Katanin 60, suggesting that Patronin is inhibitory to the severing function of Spas at the minus end. Patronin RNAi phenotype is also recovered by overexpressing Death-associated inhibitor of apoptosis 1 (Diap1), a Yorkie target gene. Heterozygote mutations in Hippo pathway genes, including hippo and warts (wts), suppress the Patronin RNAi wing phenotypes. Furthermore, Patronin physically interacts with Merlin and Expanded while reducing their function. Patronin and Klp10A antagonistically regulate their levels. Wing phenotypes of Patronin RNAi are rescued by knockdown of Klp10A, consistent with their antagonistic interaction. Klp10A overexpression also causes organ size reduction that is partially suppressed by Diap1 overexpression or wts heterozygote mutation. Taken together, this study suggests that the antagonistic interaction between Patronin and Klp10A is required for controlling cell survival and organ size by modulating microtubule stability and Hippo components. |
| 巻・号 | 28(1) |
| ページ | 233-250 |
| 公開日 | 2021-1-1 |
| DOI | 10.1038/s41418-020-0597-x |
| PII | 10.1038/s41418-020-0597-x |
| PMID | 32737445 |
| PMC | PMC7853147 |
| MeSH | Animals Cell Survival Drosophila Proteins / antagonists & inhibitors Drosophila Proteins / metabolism* Drosophila melanogaster / physiology* Female Inhibitor of Apoptosis Proteins / metabolism Intracellular Signaling Peptides and Proteins / metabolism* Kinesins / antagonists & inhibitors Kinesins / metabolism* Male Microtubule-Associated Proteins / antagonists & inhibitors Microtubule-Associated Proteins / metabolism* Microtubules / metabolism Organ Size Protein Serine-Threonine Kinases / metabolism* Signal Transduction |
| IF | 8.086 |
| 引用数 | 1 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 13 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ショウジョウバエ | 4608R-2 DGRC#116410 |