RRC ID 6084
Author Nair SK, Wang N, Turuspekov Y, Pourkheirandish M, Sinsuwongwat S, Chen G, Sameri M, Tagiri A, Honda I, Watanabe Y, Kanamori H, Wicker T, Stein N, Nagamura Y, Matsumoto T, Komatsuda T.
Title Cleistogamous flowering in barley arises from the suppression of microRNA-guided HvAP2 mRNA cleavage.
Journal Proc Natl Acad Sci U S A
Abstract The cleistogamous flower sheds its pollen before opening, forcing plants with this habit to be almost entirely autogamous. Cleistogamy also provides a means of escape from cereal head blight infection and minimizes pollen-mediated gene flow. The lodicule in cleistogamous barley is atrophied. We have isolated cleistogamy 1 (Cly1) by positional cloning and show that it encodes a transcription factor containing two AP2 domains and a putative microRNA miR172 targeting site, which is an ortholog of Arabidopsis thaliana AP2. The expression of Cly1 was concentrated within the lodicule primordia. We established a perfect association between a synonymous nucleotide substitution at the miR172 targeting site and cleistogamy. Cleavage of mRNA directed by miR172 was detectable only in a noncleistogamous background. We conclude that the miR172-derived down-regulation of Cly1 promotes the development of the lodicules, thereby ensuring noncleistogamy, although the single nucleotide change at the miR172 targeting site results in the failure of the lodicules to develop properly, producing the cleistogamous phenotype.
Volume 107(1)
Pages 490-5
Published 2010-1-5
DOI 10.1073/pnas.0909097107
PII 0909097107
PMID 20018663
PMC PMC2806734
MeSH Base Sequence Flowers / physiology* Gene Expression Regulation, Plant Hordeum / anatomy & histology Hordeum / genetics Hordeum / physiology* MicroRNAs / genetics MicroRNAs / metabolism* Molecular Sequence Data Phylogeny Plant Proteins / genetics* Plant Proteins / metabolism Polymorphism, Genetic RNA, Messenger / genetics RNA, Messenger / metabolism* RNA, Plant / genetics RNA, Plant / metabolism Sequence Alignment Transcription Factors / genetics* Transcription Factors / metabolism
IF 9.412
Times Cited 126
Barley OUH602