論文 - 詳細
| RRC ID | 60862 |
|---|---|
| 著者 | Sasaki K, Kojitani N, Hirose H, Yoshihama Y, Suzuki H, Shimada M, Takayanagi A, Yamashita A, Nakaya MA, Hirano H, Takahashi H, Ohno S. |
| タイトル | Shank2 Binds to aPKC and Controls Tight Junction Formation with Rap1 Signaling during Establishment of Epithelial Cell Polarity. |
| ジャーナル | Cell Rep |
| Abstract |
Epithelial cells establish apicobasal polarity by forming tight junctions (TJs) at the apical-lateral boundary, which play fundamental roles in physiological functions. An evolutionarily conserved atypical protein kinase C (aPKC)-partitioning defective (PAR) complex functions as a platform for TJ assembly during cell polarity establishment. However, how this complex converts the spatial cues into a subsequent active unit is unclear. Here, we identify an epithelial isoform of Shank2 as a mediator of the aPKC-PAR complex. Shank2 binds to and colocalizes with aPKC at apical junctional regions of polarized epithelial cells. Shank2 knockdown results in defects in TJ formation. Mechanistically, we find that the N-terminal SPN domain is required for the junctional localization of Shank2 and binds to the active form of Rap1 small GTPase, which is involved in TJ formation. Our findings suggest that a close physical and functional relationship between aPKC and Shank2-active Rap1 signaling serves as the platform for TJ assembly to regulate epithelial cell polarity. |
| 巻・号 | 31(1) |
| ページ | 107407 |
| 公開日 | 2020-4-7 |
| DOI | 10.1016/j.celrep.2020.02.088 |
| PII | S2211-1247(20)30262-X |
| PMID | 32268103 |
| MeSH | Adaptor Proteins, Signal Transducing / metabolism Animals Caco-2 Cells Carrier Proteins / metabolism Cell Adhesion Molecules / metabolism Cell Cycle Proteins / metabolism Cell Line Cell Polarity / physiology Dogs Epithelial Cells / metabolism Female HEK293 Cells Humans MCF-7 Cells Male Mice Nerve Tissue Proteins / metabolism* Protein Kinase C / metabolism* Shelterin Complex Signal Transduction / physiology Telomere-Binding Proteins / metabolism* Tight Junctions / metabolism |
| IF | 8.109 |
| 引用数 | 0 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 9 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 遺伝子材料 | pCMV-VSV-G-RSV-Rev (RDB04393) pCAG-HIVgp (RDB04394) |