RRC ID 60886
Author Tatebe H, Lim CT, Konno H, Shiozaki K, Shinohara A, Uchihashi T, Furukohri A.
Title Rad50 zinc hook functions as a constitutive dimerization module interchangeable with SMC hinge.
Journal Nat Commun
Abstract The human Mre11/Rad50 complex is one of the key factors in genome maintenance pathways. Previous nanoscale imaging by atomic force microscopy (AFM) showed that the ring-like structure of the human Mre11/Rad50 complex transiently opens at the zinc hook of Rad50. However, imaging of the human Mre11/Rad50 complex by high-speed AFM shows that the Rad50 coiled-coil arms are consistently bridged by the dimerized hooks while the Mre11/Rad50 ring opens by disconnecting the head domains; resembling other SMC proteins such as cohesin or condensin. These architectural features are conserved in the yeast and bacterial Mre11/Rad50 complexes. Yeast strains harboring the chimeric Mre11/Rad50 complex containing the SMC hinge of bacterial condensin MukB instead of the RAD50 hook properly functions in DNA repair. We propose that the basic role of the Rad50 hook is similar to that of the SMC hinge, which serves as rather stable dimerization interface.
Volume 11(1)
Pages 370
Published 2020-1-17
DOI 10.1038/s41467-019-14025-0
PII 10.1038/s41467-019-14025-0
PMID 31953386
PMC PMC6969161
MeSH Acid Anhydride Hydrolases / chemistry* Acid Anhydride Hydrolases / metabolism Adenosine Triphosphatases Animals Cell Cycle Proteins / chemistry* Cell Cycle Proteins / metabolism Chromosomal Proteins, Non-Histone / chemistry* Chromosomal Proteins, Non-Histone / metabolism DNA Breaks, Double-Stranded DNA Repair DNA-Binding Proteins / chemistry* DNA-Binding Proteins / metabolism Dimerization* Escherichia coli / metabolism Homologous Recombination Humans MRE11 Homologue Protein / chemistry MRE11 Homologue Protein / metabolism Microscopy, Atomic Force Multiprotein Complexes Nuclear Proteins / chemistry Nuclear Proteins / metabolism Protein Binding Protein Conformation Saccharomyces cerevisiae Saccharomyces cerevisiae Proteins / metabolism Sf9 Cells Zinc / metabolism*
IF 12.121
Times Cited 1
Yeast FY32724 FY32725