| RRC ID |
60892
|
| Author |
Aso H, Nagaoka S, Kawakami E, Ito J, Islam S, Tan BJY, Nakaoka S, Ashizaki K, Shiroguchi K, Suzuki Y, Satou Y, Koyanagi Y, Sato K.
|
| Title |
Multiomics Investigation Revealing the Characteristics of HIV-1-Infected Cells In Vivo.
|
| Journal |
Cell Rep
|
| Abstract |
For eradication of HIV-1 infection, it is important to elucidate the detailed features and heterogeneity of HIV-1-infected cells in vivo. To reveal multiple characteristics of HIV-1-producing cells in vivo, we use a hematopoietic-stem-cell-transplanted humanized mouse model infected with GFP-encoding replication-competent HIV-1. We perform multiomics experiments using recently developed technology to identify the features of HIV-1-infected cells. Genome-wide HIV-1 integration-site analysis reveals that productive HIV-1 infection tends to occur in cells with viral integration into transcriptionally active genomic regions. Bulk transcriptome analysis reveals that a high level of viral mRNA is transcribed in HIV-1-infected cells. Moreover, single-cell transcriptome analysis shows the heterogeneity of HIV-1-infected cells, including CXCL13high cells and a subpopulation with low expression of interferon-stimulated genes, which can contribute to efficient viral spread in vivo. Our findings describe multiple characteristics of HIV-1-producing cells in vivo, which could provide clues for the development of an HIV-1 cure.
|
| Volume |
32(2)
|
| Pages |
107887
|
| Published |
2020-7-14
|
| DOI |
10.1016/j.celrep.2020.107887
|
| PII |
S2211-1247(20)30868-8
|
| PMID |
32668246
|
| MeSH |
Animals
Female
Genomics*
Green Fluorescent Proteins / metabolism
HEK293 Cells
HIV Infections / genetics*
HIV Infections / metabolism*
HIV-1 / physiology*
Humans
Male
Mice
Transcriptome / genetics
|
| IF |
8.109
|
| Times Cited |
0
|
| Resource |
| DNA material |
CSII-CMV-MCS-IRES2-Bsd (RDB04385)
pCMV-VSV-G-RSV-Rev (RDB04393)
pCAG-HIVgp (RDB04394) |
