論文 - 詳細
| RRC ID | 60962 |
|---|---|
| 著者 | Rispal J, Baron L, Beaulieu JF, Chevillard-Briet M, Trouche D, Escaffit F. |
| タイトル | The H2A.Z histone variant integrates Wnt signaling in intestinal epithelial homeostasis. |
| ジャーナル | Nat Commun |
| Abstract |
The Tip60/p400 chromatin-modifying complex, which is involved in the incorporation and post-translational modification of the H2A.Z histone variant, regulates cell proliferation and important signaling pathways, such as Wnt. Here, we study the involvement of H2A.Z in intestinal epithelial homeostasis, which is dependent on the finely-tuned equilibrium between stem cells renewal and differentiation, under the control of such pathway. We use cell models and inducible knock-out mice to study the impact of H2A.Z depletion on intestinal homeostasis. We show that H2A.Z is essential for the proliferation of human cancer and normal intestinal crypt cells and negatively controls the expression of a subset of differentiation markers, in cultured cells and mice. H2A.Z impairs the recruitment of the intestine-specific transcription factor CDX2 to chromatin, is itself a target of the Wnt pathway and thus, acts as an integrator for Wnt signaling in the control of intestinal epithelial cell fate and homeostasis. |
| 巻・号 | 10(1) |
| ページ | 1827 |
| 公開日 | 2019-4-23 |
| DOI | 10.1038/s41467-019-09899-z |
| PII | 10.1038/s41467-019-09899-z |
| PMID | 31015444 |
| PMC | PMC6478875 |
| MeSH | Animals CDX2 Transcription Factor / genetics CDX2 Transcription Factor / metabolism Caco-2 Cells Cell Differentiation / genetics Cell Proliferation / genetics Chromatin / genetics Epithelial Cells / physiology Gene Expression Regulation / physiology HCT116 Cells Histones / genetics Histones / metabolism* Homeostasis / genetics* Humans Intestinal Mucosa / cytology Intestinal Mucosa / physiology* Male Mice Mice, Inbred C57BL Mice, Knockout Protein Isoforms / genetics Protein Isoforms / metabolism Wnt Signaling Pathway / genetics* |
| IF | 12.121 |
| 引用数 | 5 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 15 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 実験動物マウス | RBRC05765 |