論文 - 詳細
| RRC ID | 61022 |
|---|---|
| 著者 | Koga T, Morotomi-Yano K, Sakugawa T, Saitoh H, Yano KI. |
| タイトル | Nanosecond pulsed electric fields induce extracellular release of chromosomal DNA and histone citrullination in neutrophil-differentiated HL-60 cells. |
| ジャーナル | Sci Rep |
| Abstract |
Nanosecond pulsed electric fields (nsPEFs) have gained attention as a novel physical stimulus for life sciences. Although cancer therapy is currently their promising application, nsPEFs have further potential owing to their ability to elicit various cellular responses. This study aimed to explore stimulatory actions of nsPEFs, and we used HL-60 cells that were differentiated into neutrophils under cultured conditions. Exposure of neutrophil-differentiated HL-60 cells to nsPEFs led to the extracellular release of chromosomal DNA, which appears to be equivalent to neutrophil extracellular traps (NETs) that serve as a host defense mechanism against pathogens. Fluorometric measurement of extracellular DNA showed that DNA extrusion was rapidly induced after nsPEF exposure and increased over time. Western blot analysis demonstrated that nsPEFs induced histone citrullination that is the hydrolytic conversion of arginine to citrulline on histones and facilitates chromatin decondensation. DNA extrusion and histone citrullination by nsPEFs were cell type-specific and Ca2+-dependent events. Taken together, these observations suggest that nsPEFs drive the mechanism for neutrophil-specific immune response without infection, highlighting a novel aspect of nsPEFs as a physical stimulus. |
| 巻・号 | 9(1) |
| ページ | 8451 |
| 公開日 | 2019-6-11 |
| DOI | 10.1038/s41598-019-44817-9 |
| PII | 10.1038/s41598-019-44817-9 |
| PMID | 31186478 |
| PMC | PMC6559984 |
| MeSH | Apoptosis / genetics Apoptosis / radiation effects* Cell Differentiation / radiation effects* Chromatin / genetics Chromatin / radiation effects Citrullination / genetics Citrullination / radiation effects DNA / genetics DNA / radiation effects Electric Stimulation* Extracellular Traps / genetics Extracellular Traps / radiation effects HL-60 Cells HeLa Cells Histones / genetics Histones / radiation effects Humans Leukopoiesis / genetics Leukopoiesis / radiation effects Neutrophils / radiation effects* |
| IF | 3.998 |
| リソース情報 | |
| ヒト・動物細胞 | THP-1(RCB1189) HL60(RCB0041) Jurkat(RCB0806) K562(RCB0027) |