論文 - 詳細
| RRC ID | 61040 |
|---|---|
| 著者 | Murari A, Rhooms SK, Goparaju NS, Villanueva M, Owusu-Ansah E. |
| タイトル | An antibody toolbox to track complex I assembly defines AIF's mitochondrial function. |
| ジャーナル | J Cell Biol |
| Abstract |
An ability to comprehensively track the assembly intermediates (AIs) of complex I (CI) biogenesis in Drosophila will enable the characterization of the precise mechanism(s) by which various CI regulators modulate CI assembly. Accordingly, we generated 21 novel antibodies to various mitochondrial proteins and used this resource to characterize the mechanism by which apoptosis-inducing factor (AIF) regulates CI biogenesis by tracking the AI profile observed when AIF expression is impaired. We find that when the AIF-Mia40 translocation complex is disrupted, the part of CI that transfers electrons to ubiquinone is synthesized but fails to progress in the CI biosynthetic pathway. This is associated with a reduction in intramitochondrial accumulation of the Mia40 substrate, MIC19. Importantly, knockdown of either MIC19 or MIC60, components of the mitochondrial contact site and cristae organizing system (MICOS), fully recapitulates the AI profile observed when AIF is inhibited. Thus, AIF's effect on CI assembly is principally due to compromised intramitochondrial transport of the MICOS complex. |
| 巻・号 | 219(10) |
| 公開日 | 2020-10-5 |
| DOI | 10.1083/jcb.202001071 |
| PII | 152090 |
| PMID | 32936885 |
| PMC | PMC7659709 |
| MeSH | Animals Apoptosis Inducing Factor / genetics* Drosophila melanogaster / genetics Electron Transport Complex I / genetics Mitochondria / genetics* Mitochondrial Membrane Transport Proteins / genetics* Mitochondrial Membranes / metabolism Mitochondrial Proteins / genetics* Protein Binding / genetics |
| IF | 8.811 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 22 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ショウジョウバエ | 8680R-3 9172R-2 9350R-2 6455R-2 2LG-0618 |