論文 - 詳細
| RRC ID | 611 |
|---|---|
| 著者 | Tambasco-Studart M, Titiz O, Raschle T, Forster G, Amrhein N, Fitzpatrick TB. |
| タイトル | Vitamin B6 biosynthesis in higher plants. |
| ジャーナル | Proc Natl Acad Sci U S A |
| Abstract |
Vitamin B6 is an essential metabolite in all organisms. It can act as a coenzyme for numerous metabolic enzymes and has recently been shown to be a potent antioxidant. Plants and microorganisms have a de novo biosynthetic pathway for vitamin B6, but animals must obtain it from dietary sources. In Escherichia coli, it is known that the vitamin is derived from deoxyxylulose 5-phosphate (an intermediate in the nonmevalonate pathway of isoprenoid biosynthesis) and 4-phosphohydroxy-l-threonine. It has been assumed that vitamin B6 is synthesized in the same way in plants, but this hypothesis has never been experimentally proven. Here, we show that, in plants, synthesis of the vitamin takes an entirely different route, which does not involve deoxyxylulose 5-phosphate but instead utilizes intermediates from the pentose phosphate pathway, i.e., ribose 5-phosphate or ribulose 5-phosphate, and from glycolysis, i.e., dihydroxyacetone phosphate or glyceraldehyde 3-phosphate. The revelation is based on the recent discovery that, in bacteria and fungi, a novel pathway is in place that involves two genes (PDX1 and PDX2), neither of which is homologous to any of those involved in the previously doctrined E. coli pathway. We demonstrate that Arabidopsis thaliana has two functional homologs of PDX1 and a single homolog of PDX2. Furthermore, and contrary to what was inferred previously, we show that the pathway appears to be cytosolic and is not localized to the plastid. Last, we report that the single PDX2 homolog is essential for plant viability. |
| 巻・号 | 102(38) |
| ページ | 13687-92 |
| 公開日 | 2005-9-20 |
| DOI | 10.1073/pnas.0506228102 |
| PII | 0506228102 |
| PMID | 16157873 |
| PMC | PMC1224648 |
| MeSH | Amino Acid Sequence Arabidopsis / enzymology* Arabidopsis / genetics Arabidopsis Proteins / genetics Arabidopsis Proteins / metabolism* Carbon-Nitrogen Lyases Cytoplasm / enzymology* Escherichia coli / enzymology Escherichia coli / genetics Escherichia coli Proteins / genetics Escherichia coli Proteins / metabolism Fungal Proteins / genetics Fungal Proteins / metabolism Fungi / enzymology Fungi / genetics Glycolysis / physiology Molecular Sequence Data Nitrogenous Group Transferases / genetics Nitrogenous Group Transferases / metabolism* Pentose Phosphate Pathway / physiology Pentosephosphates / genetics Pentosephosphates / metabolism Vitamin B 6 / biosynthesis* Vitamin B 6 / genetics |
| IF | 9.412 |
| 引用数 | 128 |
| WOS 分野 | BIOCHEMISTRY & MOLECULAR BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 8 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| シロイヌナズナ / 植物培養細胞・遺伝子 | pst00965 |