RRC ID 61118
著者 Asayama S, Sakata M, Kawakami H.
タイトル Structure-activity relationship between Zn2+-chelated alkylated poly(1-vinylimidazole) and gene transfection.
ジャーナル J Inorg Biochem
Abstract The structure-activity relationship between Zn2+-chelated alkylated poly(1-vinylimidazole) (PVIm) and gene transfection has been demonstrated. From a chemical structure perspective, ethylated PVIm (PVIm-Et) chelated the most Zn2+ ions compared to methylated PVIm (PVIm-Me) and butylated PVIm (PVIm-Bu). The resulting Zn2+-chelated PVIm-Et formed more stable complexes with plasmid DNA complex than non-chelated PVIm-Et. From a biological activity perspective, the Zn2+-chelated PVIm-Et delivered the highest amount of Zn2+ ions inside the cell, corresponding to the highest gene transfection. These results suggest that PVIm-Et is the optimal sequence for the chelation of Zn2+ ions to enhance the gene transfection activity. The structure-activity relationship in this study is expected to offer a unique molecular design for drug/gene delivery systems.
巻・号 173
ページ 120-125
公開日 2017-8-1
DOI 10.1016/j.jinorgbio.2017.05.007
PII S0162-0134(16)30424-X
PMID 28511062
MeSH Alkylating Agents / chemistry* Chelating Agents / chemistry* Imidazoles / chemistry* Polyvinyls / chemistry* Structure-Activity Relationship Transfection Zinc / chemistry*
IF 3.212
リソース情報
ヒト・動物細胞 Hep G2(RCB1648)