| RRC ID |
61118
|
| Author |
Asayama S, Sakata M, Kawakami H.
|
| Title |
Structure-activity relationship between Zn2+-chelated alkylated poly(1-vinylimidazole) and gene transfection.
|
| Journal |
J Inorg Biochem
|
| Abstract |
The structure-activity relationship between Zn2+-chelated alkylated poly(1-vinylimidazole) (PVIm) and gene transfection has been demonstrated. From a chemical structure perspective, ethylated PVIm (PVIm-Et) chelated the most Zn2+ ions compared to methylated PVIm (PVIm-Me) and butylated PVIm (PVIm-Bu). The resulting Zn2+-chelated PVIm-Et formed more stable complexes with plasmid DNA complex than non-chelated PVIm-Et. From a biological activity perspective, the Zn2+-chelated PVIm-Et delivered the highest amount of Zn2+ ions inside the cell, corresponding to the highest gene transfection. These results suggest that PVIm-Et is the optimal sequence for the chelation of Zn2+ ions to enhance the gene transfection activity. The structure-activity relationship in this study is expected to offer a unique molecular design for drug/gene delivery systems.
|
| Volume |
173
|
| Pages |
120-125
|
| Published |
2017-8-1
|
| DOI |
10.1016/j.jinorgbio.2017.05.007
|
| PII |
S0162-0134(16)30424-X
|
| PMID |
28511062
|
| MeSH |
Alkylating Agents / chemistry*
Chelating Agents / chemistry*
Imidazoles / chemistry*
Polyvinyls / chemistry*
Structure-Activity Relationship
Transfection
Zinc / chemistry*
|
| IF |
3.212
|
| Resource |
| Human and Animal Cells |
Hep G2(RCB1648) |
