論文 - 詳細
| RRC ID | 6112 |
|---|---|
| 著者 | Oikawa S, Yamada T, Minohata T, Kobayashi H, Furukawa A, Tada-Oikawa S, Hiraku Y, Murata M, Kikuchi M, Yamashima T. |
| タイトル | Proteomic identification of carbonylated proteins in the monkey hippocampus after ischemia-reperfusion. |
| ジャーナル | Free Radic Biol Med |
| Abstract |
Reactive oxygen species (ROS) are known to participate in neurodegeneration after ischemia-reperfusion. With the aid of ROS, the calpain-induced lysosomal rupture provokes ischemic neuronal death in the cornu Ammonis (CA) 1 of the hippocampus; however, the target proteins of ROS still remain unknown. Here a proteomic analysis was done to identify and characterize ROS-induced carbonyl modification of proteins in the CA1 of the macaque monkey after transient whole-brain ischemia followed by reperfusion. We found that carbonyl modification of heat shock 70-kDa protein 1 (Hsp70-1), a major stress-inducible member of the Hsp70 family, was extensively increased before the neuronal death in the CA1 sector, and the carbonylation site was identified to be Arg469 of Hsp70-1. The CA1 neuronal death conceivably occurs by calpain-mediated cleavage of carbonylated Hsp70 that becomes prone to proteolysis with the resultant lysosomal rupture. In addition, the carbonyl levels of dihydropyrimidinase-like 2 isoform 2, glial fibrillary acidic protein, and beta-actin were remarkably increased in the postischemic CA1. Therefore, ischemia-reperfusion-induced oxidative damage to these proteins in the CA1 may lead to loss of the neuroprotective function, which contributes to neuronal death. |
| 巻・号 | 46(11) |
| ページ | 1472-7 |
| 公開日 | 2009-6-1 |
| DOI | 10.1016/j.freeradbiomed.2009.02.029 |
| PII | S0891-5849(09)00112-9 |
| PMID | 19272443 |
| MeSH | Actins / genetics Actins / metabolism* Animals Calpain / metabolism Cell Death Glial Fibrillary Acidic Protein / genetics Glial Fibrillary Acidic Protein / metabolism* HSP72 Heat-Shock Proteins / metabolism* Hippocampus Intercellular Signaling Peptides and Proteins / genetics Intercellular Signaling Peptides and Proteins / metabolism* Macaca Nerve Tissue Proteins / genetics Nerve Tissue Proteins / metabolism* Neurons / metabolism* Neurons / pathology Protein Carbonylation Protein Isoforms Proteomics Reactive Oxygen Species Reperfusion Injury |
| IF | 6.17 |
| 引用数 | 59 |
| WOS 分野 | ENDOCRINOLOGY & METABOLISM BIOCHEMISTRY & MOLECULAR BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 2 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ニホンザル | |