論文 - 詳細
| RRC ID | 61127 |
|---|---|
| 著者 | Murakami T, Saitoh I, Sato M, Inada E, Soda M, Oda M, Domon H, Iwase Y, Sawami T, Matsueda K, Terao Y, Ohshima H, Noguchi H, Hayasaki H. |
| タイトル | Isolation and characterization of lymphoid enhancer factor-1-positive deciduous dental pulp stem-like cells after transfection with a piggyBac vector containing LEF1 promoter-driven selection markers. |
| ジャーナル | Arch Oral Biol |
| Abstract |
OBJECTIVE:Lymphoid enhancer-binding factor-1 (LEF1) is a 48-kD nuclear protein that is expressed in pre-B and T cells. LEF1 is also an important member of the Wnt/β-catenin signaling pathway that plays important roles in the self-renewal and differentiation of embryonic stem cells. We speculated that LEF1 might function in the stem cells from human exfoliated deciduous teeth (SHED). In this study, we attempted to isolate such LEF1-positive cells from human deciduous dental pulp cells (HDDPCs) by genetic engineering technology, using the human LEF1 promoter. DESIGN:A piggyBac transposon plasmid (pTA-LEN) was introduced into HDDPCs, using the Neon® transfection system. After G418 selection, the emerging colonies were assessed for EGFP-derived fluorescence by fluorescence microscopy. Reverse transcription polymerase chain reaction (RT-PCR) analysis was performed using RNA isolated from these colonies to examine stem cell-specific transcript expression. Osteoblastic or neuronal differentiation was induced by cultivating the LEF1-positive cells with differentiation-inducing medium. RESULTS:RT-PCR analysis confirmed the expression of several stem cell markers, including OCT3/4, SOX2, REX1, and NANOG, in LEF1-positive HDDPCs, which could be differentiated into osteoblasts and neuronal cells. CONCLUSIONS:The isolated LEF1-positive HDDPCs exhibited the properties of stem cells, suggesting that LEF1 might serve as a marker for SHED. |
| 巻・号 | 81 |
| ページ | 110-120 |
| 公開日 | 2017-9-1 |
| DOI | 10.1016/j.archoralbio.2017.04.033 |
| PII | S0003-9969(17)30143-7 |
| PMID | 28500952 |
| MeSH | Biomarkers / metabolism Cell Differentiation Cells, Cultured DNA Transposable Elements* Dental Pulp / cytology* Humans Induced Pluripotent Stem Cells / cytology* Lymphoid Enhancer-Binding Factor 1 / metabolism* Nerve Tissue Proteins / metabolism Osteoblasts / cytology Promoter Regions, Genetic Reverse Transcriptase Polymerase Chain Reaction Tooth, Deciduous Transfection |
| IF | 1.931 |
| リソース情報 | |
| ヒト・動物細胞 | RAJI(RCB1647) |