RRC ID 61133
Author Negi S, Terada Y, Suzuyama M, Matsumoto H, Honbo A, Amagase Y, Mizukawa Y, Kiriyama A, Iga K, Urushidaini T, Sugiura Y.
Title Intrinsic cell permeability of the GAGA zinc finger protein into HeLa cells.
Journal Biochem Biophys Res Commun
Abstract We examined the intrinsic cell permeability of a GAGA zinc finger obtained from the Drosophila melanogaster transcription factor and analyzed its mechanism of cellular uptake using confocal microscopy and flow cytometry. HeLa cells were treated with the Cy5-labeld GAGA peptides (containing a fluorescent chromophore) to detect fluorescence signals from the fluorescent labeling peptides by confocal microscopy. The results clearly indicated that GAGA peptides possess intrinsic cell permeability for HeLa cells. Based on the results of the flow cytometry analysis and the theoretical net positive charge of the GAGA peptides, the efficiency of cellular uptake of the GAGA peptides was predicted to depend on the net positive charge of the GAGA peptide as well as the cationic component ratio of Arg residues to Lys residues.
Volume 464(4)
Pages 1034-1039
Published 2015-9-4
DOI 10.1016/j.bbrc.2015.07.060
PII S0006-291X(15)30290-4
PMID 26187668
MeSH Amino Acid Sequence Animals Arginine / chemistry Cell Membrane Permeability DNA-Binding Proteins / chemistry DNA-Binding Proteins / genetics DNA-Binding Proteins / metabolism* Drosophila Proteins / chemistry Drosophila Proteins / genetics Drosophila Proteins / metabolism* Flow Cytometry HeLa Cells Humans Lysine / chemistry Microscopy, Confocal Molecular Sequence Data Peptide Fragments / chemistry Peptide Fragments / genetics Peptide Fragments / metabolism Recombinant Proteins / chemistry Recombinant Proteins / genetics Recombinant Proteins / metabolism Static Electricity Transcription Factors / chemistry Transcription Factors / genetics Transcription Factors / metabolism* Zinc Fingers / genetics Zinc Fingers / physiology*
Human and Animal Cells HeLa(RCB0007)