論文 - 詳細
| RRC ID | 61145 |
|---|---|
| 著者 | Takii R, Fujimoto M, Matsumoto M, Srivastava P, Katiyar A, Nakayama KI, Nakai A. |
| タイトル | The pericentromeric protein shugoshin 2 cooperates with HSF1 in heat shock response and RNA Pol II recruitment. |
| ジャーナル | EMBO J |
| Abstract |
The recruitment of RNA polymerase II (Pol II) to core promoters is highly regulated during rapid induction of genes. In response to heat shock, heat shock transcription factor 1 (HSF1) is activated and occupies heat shock gene promoters. Promoter-bound HSF1 recruits general transcription factors and Mediator, which interact with Pol II, but stress-specific mechanisms of Pol II recruitment are unclear. Here, we show in comparative analyses of HSF1 paralogs and their mutants that HSF1 interacts with the pericentromeric adaptor protein shugoshin 2 (SGO2) during heat shock in mouse cells, in a manner dependent on inducible phosphorylation of HSF1 at serine 326, and recruits SGO2 to the HSP70 promoter. SGO2-mediated binding and recruitment of Pol II with a hypophosphorylated C-terminal domain promote expression of HSP70, implicating SGO2 as one of the coactivators that facilitate Pol II recruitment by HSF1. Furthermore, the HSF1-SGO2 complex supports cell survival and maintenance of proteostasis in heat shock conditions. These results exemplify a proteotoxic stress-specific mechanism of Pol II recruitment, which is triggered by phosphorylation of HSF1 during the heat shock response. |
| 巻・号 | 38(24) |
| ページ | e102566 |
| 公開日 | 2019-12-16 |
| DOI | 10.15252/embj.2019102566 |
| PMID | 31657478 |
| PMC | PMC6912021 |
| MeSH | Adaptor Proteins, Signal Transducing / genetics Adaptor Proteins, Signal Transducing / metabolism Animals Cell Cycle Proteins / genetics Cell Cycle Proteins / metabolism* Gene Expression Regulation Gene Knockdown Techniques HSP70 Heat-Shock Proteins / genetics HSP70 Heat-Shock Proteins / metabolism Heat Shock Transcription Factors / metabolism* Heat-Shock Response / genetics Heat-Shock Response / physiology* Mice Mice, Knockout Phosphorylation Protein Binding RNA Polymerase II / metabolism* |
| IF | 9.889 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 14 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 遺伝子材料 | pShuttle-CMV/mSGO2-HA (RDB18205) pShuttle-CMV/cHSF1-Flag (RDB18206) pShuttle-CMV/AsHSF1-Flag (RDB18207) |