RRC ID 61145
著者 Takii R, Fujimoto M, Matsumoto M, Srivastava P, Katiyar A, Nakayama KI, Nakai A.
タイトル The pericentromeric protein shugoshin 2 cooperates with HSF1 in heat shock response and RNA Pol II recruitment.
ジャーナル EMBO J
Abstract The recruitment of RNA polymerase II (Pol II) to core promoters is highly regulated during rapid induction of genes. In response to heat shock, heat shock transcription factor 1 (HSF1) is activated and occupies heat shock gene promoters. Promoter-bound HSF1 recruits general transcription factors and Mediator, which interact with Pol II, but stress-specific mechanisms of Pol II recruitment are unclear. Here, we show in comparative analyses of HSF1 paralogs and their mutants that HSF1 interacts with the pericentromeric adaptor protein shugoshin 2 (SGO2) during heat shock in mouse cells, in a manner dependent on inducible phosphorylation of HSF1 at serine 326, and recruits SGO2 to the HSP70 promoter. SGO2-mediated binding and recruitment of Pol II with a hypophosphorylated C-terminal domain promote expression of HSP70, implicating SGO2 as one of the coactivators that facilitate Pol II recruitment by HSF1. Furthermore, the HSF1-SGO2 complex supports cell survival and maintenance of proteostasis in heat shock conditions. These results exemplify a proteotoxic stress-specific mechanism of Pol II recruitment, which is triggered by phosphorylation of HSF1 during the heat shock response.
巻・号 38(24)
ページ e102566
公開日 2019-12-16
DOI 10.15252/embj.2019102566
PMID 31657478
PMC PMC6912021
MeSH Adaptor Proteins, Signal Transducing / genetics Adaptor Proteins, Signal Transducing / metabolism Animals Cell Cycle Proteins / genetics Cell Cycle Proteins / metabolism* Gene Expression Regulation Gene Knockdown Techniques HSP70 Heat-Shock Proteins / genetics HSP70 Heat-Shock Proteins / metabolism Heat Shock Transcription Factors / metabolism* Heat-Shock Response / genetics Heat-Shock Response / physiology* Mice Mice, Knockout Phosphorylation Protein Binding RNA Polymerase II / metabolism*
IF 9.889
リソース情報
遺伝子材料 pShuttle-CMV/mSGO2-HA (RDB18205) pShuttle-CMV/cHSF1-Flag (RDB18206) pShuttle-CMV/AsHSF1-Flag (RDB18207)