Reference - Detail
| RRC ID | 61162 |
|---|---|
| Author | Itaya M, Shaheduzzaman SM, Matsui K, Omori A, Tsuji T. |
| Title | Green marker for colonies of Bacillus subtilis. |
| Journal | Biosci Biotechnol Biochem |
| Abstract |
A Bacillus subtilis plasmid encoding a green fluorescence protein gene (gfp) was constructed. The fluorescence of B. subtilis colonies having this plasmid on agar plates was so high that they could be readily discerned visually under UV light. The fluorescence could be effectively expressed in three ways (i) through use of a strong bsr promoter (blasticidin S resistance gene), (ii) by efficient translation with the bsr translation system, and (iii) through increase in the copy number per cell. The high stability of the GFP plasmid was demonstrated by using more complicated growth conditions without any antibiotic for selection. |
| Volume | 65(3) |
| Pages | 579-83 |
| Published | 2001-3-1 |
| DOI | 10.1271/bbb.65.579 |
| PMID | 11330671 |
| MeSH | Amino Acid Sequence Bacillus subtilis / genetics Bacillus subtilis / growth & development* Base Sequence Biomarkers DNA, Bacterial Fluorescence Gene Dosage Genes, Bacterial Green Fluorescent Proteins Luminescent Proteins / genetics Luminescent Proteins / metabolism* Molecular Sequence Data Plasmids |
| IF | 1.516 |
| Resource | |
| Prokaryotes B. subtilis | MBS769 MBS770 |