RRC ID 61162
著者 Itaya M, Shaheduzzaman SM, Matsui K, Omori A, Tsuji T.
タイトル Green marker for colonies of Bacillus subtilis.
ジャーナル Biosci Biotechnol Biochem
Abstract A Bacillus subtilis plasmid encoding a green fluorescence protein gene (gfp) was constructed. The fluorescence of B. subtilis colonies having this plasmid on agar plates was so high that they could be readily discerned visually under UV light. The fluorescence could be effectively expressed in three ways (i) through use of a strong bsr promoter (blasticidin S resistance gene), (ii) by efficient translation with the bsr translation system, and (iii) through increase in the copy number per cell. The high stability of the GFP plasmid was demonstrated by using more complicated growth conditions without any antibiotic for selection.
巻・号 65(3)
ページ 579-83
公開日 2001-3-1
DOI 10.1271/bbb.65.579
PMID 11330671
MeSH Amino Acid Sequence Bacillus subtilis / genetics Bacillus subtilis / growth & development* Base Sequence Biomarkers DNA, Bacterial Fluorescence Gene Dosage Genes, Bacterial Green Fluorescent Proteins Luminescent Proteins / genetics Luminescent Proteins / metabolism* Molecular Sequence Data Plasmids
IF 1.516
リソース情報
原核生物(枯草菌) MBS769 MBS770