RRC ID 61166
Author Kuroki A, Toda T, Matsui K, Uotsu-Tomita R, Tomita M, Itaya M.
Title Reshuffling of the Bacillus subtilis 168 genome by multifold inversion.
Journal J Biochem
Abstract The genome of Bacillus subtilis 168 was modified to yield a genome vector for the cloning of DNA several Mb in size. Unlike contemporary plasmid-based vectors, this 4.2 Mb genome vector requires specific in vivo handling protocols because of its large size. Inversion mutagenesis, a method to modify local genome structure without gain or loss of genes, was applied intensively to the B. subtilis genome; this technique made possible both exchange and translocation of designated regions of the genome. This method not only reshuffles the genome of B. subtilis, but can provide insight into the biologic principles underlying genome plasticity.
Volume 143(1)
Pages 97-105
Published 2008-1-1
DOI 10.1093/jb/mvm197
PII mvm197
PMID 17965428
MeSH Bacillus subtilis / classification Bacillus subtilis / genetics* Bacillus subtilis / isolation & purification Chromosome Inversion* DNA Shuffling Drug Resistance, Bacterial Genome, Bacterial* Mutation Neomycin / pharmacology
IF 2.23
Resource
Prokaryotes B. subtilis MBS807 MBS814 MBS815 MBS816 MBS817 MBS818 MBS819 MBS820 MBS822