RRC ID 61167
Author Itaya M.
Title Sequential insertion of multiple I-SceI recognition sites at designed loci of the Bacillus subtilis 168 genome.
Journal Biosci Biotechnol Biochem
Abstract Bacillus subtilis 168 is the only bacterium-based host serving for the cloning of giant DNA above 1.000 kbp. As rapid verification of the genome structure is crucial during the cloning process, six of 18-base sequence recognized by endonuclease I-SceI were sequentially created in the B. subtilis 168 genome. The established method and materials should be of use for other B. subtilis derivatives.
Volume 76(1)
Pages 180-2
Published 2012-1-1
DOI 10.1271/bbb.110528
PII JST.JSTAGE/bbb/110528
PMID 22232257
MeSH Bacillus subtilis / enzymology* Bacillus subtilis / genetics* Binding Sites Endonucleases / metabolism* Genetic Loci / genetics* Genome, Bacterial / genetics* Mutagenesis, Insertional*
IF 1.297
Resource
Prokaryotes B. subtilis MBS774 MBS775 MBS776 MBS777 MBS778 MBS779 MBS780 MBS781 MBS782 MBS783