論文 - 詳細
| RRC ID | 61170 |
|---|---|
| 著者 | Itaya M, Sato M, Hasegawa M, Kono N, Tomita M, Kaneko S. |
| タイトル | Far rapid synthesis of giant DNA in the Bacillus subtilis genome by a conjugation transfer system. |
| ジャーナル | Sci Rep |
| Abstract |
Bacillus subtilis offers a platform for giant DNA synthesis, which is mediated by the connection of overlapping DNA segments called domino DNA, in the cloning locus of the host. The domino method was successfully used to produce DNA fragments as large as 3500 kbp. However, domino DNA is limited to <100 kbp because of size restrictions regarding the transformation (TF) of B. subtilis competent cells. A novel conjugal transfer (CT) method was designed to eliminate the TF size limit. The CT method enables rapid and efficient domino reactions in addition to the transfer of giant DNA molecules of up to 875 kbp to another B. subtilis genome within 4 hours. The combined use of the TF and CT should enable significantly rapid giant DNA production. |
| 巻・号 | 8(1) |
| ページ | 8792 |
| 公開日 | 2018-6-8 |
| DOI | 10.1038/s41598-018-26987-0 |
| PII | 10.1038/s41598-018-26987-0 |
| PMID | 29884789 |
| PMC | PMC5993740 |
| MeSH | Bacillus subtilis / genetics* Cloning, Molecular / methods DNA / genetics* DNA Replication Genome, Bacterial Transformation, Genetic* |
| IF | 3.998 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 原核生物(枯草菌) | MBS757 MBS758 MBS759 MBS760 MBS761 MBS762 MBS763 MBS764 MBS766 MBS767 |