| RRC ID |
61171
|
| Author |
Itaya M, Sato M, Watanabe S, Yoshikawa H, Tomita M, Sato R.
|
| Title |
Stable mutants of restriction-deficient/modification-proficient Bacillus subtilis 168: hub strains for giant DNA engineering.
|
| Journal |
J Biochem
|
| Abstract |
Bacillus subtilis 168 has been explored as a platform for the synthesis and transmission of large DNA. Two inherent DNA incorporation systems, natural transformation and pLS20-based conjugation transfer, enable rapid handling of target DNA. Both systems are affected by the Bsu restriction-modification system that recognizes and cleaves unmethylated XhoI sites, limiting the choice of target DNA. We constructed B. subtilis 168 with stable mutation for restriction-deficient and modification-proficient (r-m+). It was demonstrated that the r-m+ strains can incorporate and transfer synthesized DNA with multiple XhoI sites. These should be of value as hub strains to integrate and disseminate giant DNA between B. subtilis 168 derivatives.
|
| Volume |
166(3)
|
| Pages |
231-236
|
| Published |
2019-9-1
|
| DOI |
10.1093/jb/mvz031
|
| PII |
5475605
|
| PMID |
31004491
|
| MeSH |
Bacillus subtilis / genetics*
DNA, Bacterial / genetics*
Genetic Engineering*
Mutation
|
| IF |
2.476
|
| Resource |
| Prokaryotes B. subtilis |
MBS829
MBS830
MBS831
MBS832
MBS833
MBS834
MBS835
MBS836
MBS837
MBS838
MBS839 |
