RRC ID 61179
著者 Nagao A, Maoka T, Ono H, Kotake-Nara E, Kobayashi M, Tomita M.
タイトル A 3-hydroxy β-end group in xanthophylls is preferentially oxidized to a 3-oxo ε-end group in mammals.
ジャーナル J Lipid Res
Abstract We previously found that mice fed lutein accumulated its oxidative metabolites (3'-hydroxy-ε,ε-caroten-3-one and ε,ε-carotene-3,3'-dione) as major carotenoids, suggesting that mammals can convert xanthophylls to keto-carotenoids by the oxidation of hydroxyl groups. Here we elucidated the metabolic activities of mouse liver for several xanthophylls. When lutein was incubated with liver postmitochondrial fraction in the presence of NAD(+), (3'R,6'R)-3'-hydroxy-β,ε-caroten-3-one and (6RS,3'R,6'R)-3'-hydroxy-ε,ε-caroten-3-one were produced as major oxidation products. The former accumulated only at the early stage and was assumed to be an intermediate, followed by isomerization to the latter. The configuration at the C3' and C6' of the ε-end group in lutein was retained in the two oxidation products. These results indicate that the 3-hydroxy β-end group in lutein was preferentially oxidized to a 3-oxo ε-end group via a 3-oxo β-end group. Other xanthophylls such as β-cryptoxanthin and zeaxanthin, which have a 3-hydroxy β-end group, were also oxidized in the same manner as lutein. These keto-carotenoids, derived from dietary xanthophylls, were confirmed to be present in plasma of normal human subjects, and β,ε-caroten-3'-one was significantly increased by the ingestion of β-cryptoxanthin. Thus, humans as well as mice have oxidative activity to convert the 3-hydroxy β-end group of xanthophylls to a 3-oxo ε-end group.
巻・号 56(2)
ページ 449-62
公開日 2015-2-1
DOI 10.1194/jlr.P055459
PII S0022-2275(20)35629-7
PMID 25502844
PMC PMC4306698
MeSH Animals Carotenoids / chemistry Carotenoids / metabolism Cryptoxanthins / chemistry Cryptoxanthins / metabolism Humans Liver / metabolism Lutein / analogs & derivatives Lutein / chemistry Lutein / metabolism Male Mammals Mice, Inbred ICR Oxidation-Reduction Xanthophylls / chemistry Xanthophylls / metabolism* Zeaxanthins / chemistry Zeaxanthins / metabolism
IF 4.483
リソース情報
ヒト・動物細胞 RAW 264(RCB0535)