論文 - 詳細
| RRC ID | 61182 |
|---|---|
| 著者 | Ozaki M, Takayama T, Yamamoto T, Ozawa Y, Nagao M, Tanabe N, Nakajima A, Suzuki N, Maeno M, Yamano S, Sato S. |
| タイトル | A collagen membrane containing osteogenic protein-1 facilitates bone regeneration in a rat mandibular bone defect. |
| ジャーナル | Arch Oral Biol |
| Abstract |
OBJECTIVES:Osteogenic protein-1 (OP-1) has shown osteoinductive activities and is useful for clinical treatments, including bone regeneration. Regenerative procedures using a bioabsorbable collagen membrane (BCM) are well established in periodontal and implant dentistry. We evaluated the subsequent effects of the BCM in combination with OP-1 on bone regeneration in a rat mandibular circular critical-sized bone defect in vivo. DESIGN:We used 8 rats that received surgery in both sides of the mandible, and created the total 16 defects which were divided into 4 groups: Group 1; no treatment, as a control, Group 2; BCM alone, Group 3; BCM containing low dose 0.5μg of OP-1 (L-OP-1), and Group 4; BCM containing high dose 2.0μg of OP-1 (H-OP-1). Newly formed bone was evaluated by micro computed tomography (micro-CT) and histological analyses at 8 weeks postoperatively. In quantitative and qualitative micro-CT analyses of the volume of new bone formation, bone density, and percentage of new bone area was evaluated. RESULTS:BCM with rhOP-1 significantly increased and accelerated bone volume, bone mineral density, and percentage of new bone area compared to control and BCM alone at 8 weeks after surgery; these enhancements in bone regeneration in the OP-1-treated groups were dose-dependent. CONCLUSIONS:OP-1 delivered with a BCM may have effective osteoinductive potency and be a good combination for bone regeneration. The use of such a combination device for osteogenesis may result in safer and more predictable bone regenerative outcomes in the future. |
| 巻・号 | 84 |
| ページ | 19-28 |
| 公開日 | 2017-12-1 |
| DOI | 10.1016/j.archoralbio.2017.09.005 |
| PII | S0003-9969(17)30276-5 |
| PMID | 28938197 |
| MeSH | Animals Bone Morphogenetic Protein 7 / pharmacology* Bone Regeneration / drug effects* Cell Survival / drug effects Cells, Cultured Collagen / pharmacology* Enzyme-Linked Immunosorbent Assay Male Mandible / diagnostic imaging Mandible / surgery* Membranes, Artificial Osteoblasts / drug effects Rats Rats, Inbred F344 X-Ray Microtomography |
| IF | 1.931 |
| リソース情報 | |
| ヒト・動物細胞 | MC3T3-E1(RCB1126) |