| RRC ID |
6119
|
| Author |
Fujii T, Kuwazaki S, Yamamoto K, Abe H, Ohnuma A, Katsuma S, Mita K, Shimada T.
|
| Title |
Identification and molecular characterization of a sex chromosome rearrangement causing a soft and pliable (spli) larval body phenotype in the silkworm, Bombyx mori.
|
| Journal |
Genome
|
| Abstract |
We carried out genetic and cytogenetic analyses of X-ray-induced deleterious Z chromosomes that result in a soft and pliable (spli) phenotype in the silkworm, Bombyx mori. In a B. mori strain with a spli phenotype, we found the Z chromosome broken between the sch (1-21.5) and od (1-49.6) loci. We also found a chromosomal fragment bearing a fifth-chromosome locus for egg and eye pigmentation fused to a Z chromosome fragment. By means of fluorescence in situ hybridization using bacterial artificial chromosome clones as probes, we confirmed that the fused chromosome is composed of a fragment of chromosome 5 and a fragment of the Z chromosome. Moreover, a predicted gene, GA002017, the Bombyx ortholog of the Drosophila gene acj6 (Bmacj6), was completely deleted by the Z chromosome breakage event. The relationship between Bmacj6 and the spli phenotype is discussed.
|
| Volume |
53(1)
|
| Pages |
45-54
|
| Published |
2010-1-1
|
| DOI |
10.1139/g09-083
|
| PII |
g09-083
|
| Description |
spli（エスプリ）の原因遺伝子はBmacj6である。Bmacj6はPOUドメインを持った転写因子をコードする。n41系統(spli/spli)のZ染色体は二つに切断されており、切断によって、Bmacj6のエクソン2～4が欠失している。
|
| PMID |
20130748
|
| MeSH |
Animals
Bombyx / anatomy & histology*
Bombyx / genetics*
Bombyx / growth & development
Chromosome Breakage
Chromosome Mapping
Cloning, Molecular
Female
Genetic Loci
Genetic Markers / physiology
Larva / anatomy & histology*
Larva / genetics*
Male
Models, Biological
Phenotype
Sequence Deletion
Sex Chromosome Aberrations*
Sex Chromosomes / genetics
Somatotypes / genetics
|
| IF |
2.037
|
| Times Cited |
4
|
|
WOS Category
|
BIOTECHNOLOGY & APPLIED MICROBIOLOGY
GENETICS & HEREDITY
|
| Resource |
| Silkworms |
p50
n41
u01 |
