RRC ID 61205
Author Osakabe K, Wada N, Miyaji T, Murakami E, Marui K, Ueta R, Hashimoto R, Abe-Hara C, Kong B, Yano K, Osakabe Y.
Title Genome editing in plants using CRISPR type I-D nuclease.
Journal Commun Biol
Abstract Genome editing in plants has advanced greatly by applying the clustered regularly interspaced short palindromic repeats (CRISPRs)-Cas system, especially CRISPR-Cas9. However, CRISPR type I-the most abundant CRISPR system in bacteria-has not been exploited for plant genome modification. In type I CRISPR-Cas systems, e.g., type I-E, Cas3 nucleases degrade the target DNA in mammals. Here, we present a type I-D (TiD) CRISPR-Cas genome editing system in plants. TiD lacks the Cas3 nuclease domain; instead, Cas10d is the functional nuclease in vivo. TiD was active in targeted mutagenesis of tomato genomic DNA. The mutations generated by TiD differed from those of CRISPR/Cas9; both bi-directional long-range deletions and short indels mutations were detected in tomato cells. Furthermore, TiD can be used to efficiently generate bi-allelic mutant plants in the first generation. These findings indicate that TiD is a unique CRISPR system that can be used for genome engineering in plants.
Volume 3(1)
Pages 648
Published 2020-11-6
DOI 10.1038/s42003-020-01366-6
PII 10.1038/s42003-020-01366-6
PMID 33159140
PMC PMC7648086
MeSH Clustered Regularly Interspaced Short Palindromic Repeats Deoxyribonucleases / genetics* Gene Editing* Genetic Engineering* Genome, Plant* Solanum lycopersicum / genetics*
IF 4.165
Tomato TOMJPF00004 (Ailsa Craig)
Human and Animal Cells 293T(RCB2202)