RRC ID 61206
Author Miura M, Tanaka K, Komatsu Y, Suda M, Yasoda A, Sakuma Y, Ozasa A, Nakao K.
Title A novel interaction between thyroid hormones and 1,25(OH)(2)D(3) in osteoclast formation.
Journal Biochem Biophys Res Commun
Abstract Thyroid hormones enhance osteoclast formation and their excess is an important cause of secondary osteoporosis. 3,5,3' -Triiodo-L-thyronine (T3) induced the mRNA expression of receptor activator of nuclear factor-kappa B ligand (RANKL), which is a key molecule in osteoclast formation, in primary osteoblastic cells (POB). This effect was amplified in the copresence of 1 alpha,25-dihydroxyvitamin D(3) (1,25(OH)(2)D(3)). Although T3 alone did not induce octeoclasts in coculture of bone marrow cells with POB, T3 enhanced 1,25(OH)(2)D(3)-induced osteoclast formation. Thyroxine (T4) also enhanced 1,25(OH)(2)D(3)-induced osteoclast formation. These data suggested that T4 was locally metabolized to T3 for its action, since T4 is a prohormone with little hormonal activity. The mRNA expression of type-2 iodothyronine deiodinase (D2), which is responsible for maintaining local T3 concentration, was induced by 1,25(OH)(2)D(3) dose- and time-dependently. Our data would facilitate our understanding of the mechanism of osteoclast formation by thyroid hormones and suggest a novel interaction between thyroid hormones and 1,25(OH)(2)D(3).
Volume 291(4)
Pages 987-94
Published 2002-3-8
DOI 10.1006/bbrc.2002.6561
PII S0006291X02965617
PMID 11866463
MeSH Animals Animals, Newborn Calcitriol / pharmacology* Carrier Proteins / biosynthesis Carrier Proteins / genetics Cell Line Cells, Cultured Coculture Techniques Dose-Response Relationship, Drug Drug Synergism Hematopoietic Stem Cells / drug effects Hematopoietic Stem Cells / physiology Iodide Peroxidase / biosynthesis Iodide Peroxidase / genetics Kinetics Membrane Glycoproteins / biosynthesis Membrane Glycoproteins / genetics Mice Osteoblasts / drug effects Osteoblasts / metabolism Osteoclasts / physiology* RANK Ligand RNA, Messenger / biosynthesis Receptor Activator of Nuclear Factor-kappa B Thyroxine / pharmacology* Triiodothyronine / pharmacology*
IF 2.985
Human and Animal Cells ST2(RCB0224)